pLenti-EF1a-C-Myc-DDK-IRES-BSD Lentiviral Gene Expression Vector

SKU PS100139

pLenti-EF1a-C-Myc-DDK-IRES-BSD, Lenti vector with bicistronic IRES-BSD, 10ug

Product Data

• C-Tag: Myc-DDK
• E. coli Selection: Chl (34 ug/mL)
• Mammalian Cell Selection: Blasticidin
• Expression Promoter: EF1a
• Features:
  • This Lenti vector is a third generation of lentiviral vector.
  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-BSD is within the Lentiviral packaging cassette and would allow Blasticidin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol(34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • WPRE_R Primer can be used to sequence the target sequence after cloning.
• Shipping: Blue Ice