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pLenti-EF1a-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

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SKU
PS100120
pLenti-EF1a-C-Myc-DDK-P2A-Puro, Lenti vector with EF1a promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter EF1a
Features

This Lenti vector is a third generation lentiviral vector.

  • The ORF cloned in this vector can be expressed as a tagged fusion protein with C-terminal Myc-DDK tag.
  • The expression of the cloned ORF is driven by EF1a promoter, a constitutive promoter.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34ug/ml)
  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states
Lama, Morozov, Garzia et al
Commun Biol (2026) 9 (1), 239 DOI: 10.1038/s42003-025-09343-7
Cited by 1
AI Summary: OriGene’s pLenti-EF1a-C-Myc-DDK-P2A-Puro empty plasmid (PS100120) was used for lentiviral particle production to facilitate gene knockout or overexpression experiments in the study.
Snippet: ansductionFour million HEK293T/17 cells were plated into a 10-cm cell culture plate. Lentiviral particle production was initiated 24 h after seeding by co-transfecting 5 µg Origene pLenti-EF1a-C-Myc-DDK-P2A-Puro empty plasmid PS100120 or pLenti-EF1a
FigureFigureFigure
TM4SF5 knockout protects mice from diet-induced obesity partly by regulating autophagy in adipose tissue
Choi, Son, Kim et al
Diabetes (2021)
Cited by 17
AI Summary: OriGene products, specifically the TM4SF5 cDNA clone (MR201934) and lentiviral vectors (PS100121 and PS100120), were used to manipulate TM4SF5 expression in cells, enabling the study of its role in autophagy and metabolism in adipose tissue.
Snippet: (MR201934, Origene, Rockville, MD) was cloned into pLenti-EF1a-C-mGFP-P2A- Puro Lentiviral Vector (PS100121, Origene) or pLenti-EF1a-C-Myc-DDK-P2A-Puro Lentiviral Vector (PS100120, Origene) using AsiSI and MluI. Lentiviral particle production and Page
Showing 1-2 of 2 papers.
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