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pLenti-P2A-Puro Lentiviral Gene Expression Vector

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SKU
PS100109
pLenti-P2A-Puro, tag free Lenti vector with P2A-Puro, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34 ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Comparative analysis of LAG3 antibodies shows differential binding patterns by flow cytometry
Graydon, Balasko, Kowatsch et al
J Immunol Methods (2024) 534, 113757 DOI: 10.1016/j.jim.2024.113757
Cited by 2
AI Summary: OriGene’s LAG3 open reading frame plasmid (cat# PS100109) was used to generate LAG3+ and LAG3- cell lines for evaluating the specificity and sensitivity of different LAG3 antibodies by flow cytometry in this study.
Snippet: , cat# PS100109) alone (LAG3-) or the same plasmid containing the full LAG3 open reading frame (LAG3+) cloned using restriction endonucleases SgfI and MluI (Origene)
Investigating the role of lectins in LAG3 function and T cell activity
Mohideen
Thesis (2024)
Snippet: ll line, were built using a third-generation lentiviral gene expression vector, pLenti-P2A-Puro (cat# PS100109;Origene). This lentiviral packaging cassette houses a P2A-Puro marker, enabling puromycin selection in transduced cells
Platelet Endothelial Cell Adhesion Molecule 1 (CD31) Is Essential for Clostridium perfringens Beta-Toxin Mediated Cytotoxicity in Human Endothelial and Monocytic Cells
Tarek, Bruggisser, Cattalani et al
Toxins (Basel) (2021) 13 (12) DOI: 10.3390/toxins13120893
Cited by 12
AI Summary: OriGene’s pLenti-P2A-Puro plasmid (#PS100109), modified to create pLenti-P2A-GFP-Blast, was used to express mutated human CD31 in lentiviral transduction experiments, enabling the study of CD31’s role in Clostridium perfringens beta-toxin cytotoxicity.
Snippet: … The modified sequences were synthesized and cloned into pLenti-P2A-GFP-Blast plasmid (modified from pLenti-P2A-Puro, Origene #PS100109). … After t;(modified from pLenti-P2A-Puro, Origene #PS100109)
FigureFigureFigure
HIV-based lentiviral vectors: origin and sequence differences
Johnson, Alvarado, Moffatt et al
Mol Ther Methods Clin Dev (2021) 21, 451-465 DOI: 10.1016/j.omtm.2021.03.018
Cited by 43
AI Summary: In the paper "HIV-based lentiviral vectors: origin and sequence differences," OriGene products—specifically, the HIV-1 NL4-3 and HXB2 reference plasmids—were used as source material for sequence comparison and phylogenetic analysis of lentiviral vector backbones.
FigureFigureFigure
HIV-based lentiviral vectors: origin and sequence differences
Johnson, Alvarado, Moffatt et al
Molecular Therapy - Methods & Clinical Development (2021) 21, 451-465 DOI: 10.1016/j.omtm.2021.03.018
Cited by 43
AI Summary: In this study, the OriGene pLenti vector (cat #PS100109) was included among several lentiviral vector backbones analyzed for sequence differences and homology, contributing to the comparative evaluation of clinically relevant HIV-based lentiviral vector systems.
Snippet: 1422);93 pLK0.1 (Addgene;cat #10878);94 pLL3.7 m (Addgene;cat #89362);95 Puro.cre (Addgene;cat #17408);96 pRSIEG (Cellecta;SVSHU6EG-L);pLenti7.3 (Thermo Fisher Scientific;cat #V53406);pLenti (OriGene;cat #PS100109);pSF_Lenti (Sigma;cat #OGS269);pLV-GFPSpark (Sinobiological;cat #LVCV-01);pLVX (Takara;cat #632164);and pLenti (Vigene;cat #P100020). …Swaminathan, Stephen E. Braun PII: S2329-0501(21)00058-9;pRSIEG (Cellecta SVSHU6EG-L), pLenti7.3 (Thermo Cat# V53406), pLenti (Origene,Cat#PS100109), pSF_Lenti (Sigma Cat#OGS269),
SP1-induced long non-coding RNA SNHG6 facilitates the carcinogenesis of chondrosarcoma through inhibiting KLF6 by recruiting EZH2
Pu, Shi, Chen et al
Cell Death Dis (2021) 12 (1), 59 DOI: 10.1038/s41419-020-03352-6
Cited by 22
AI Summary: In this study, the pLenti-P2A-Puro expression plasmid from OriGene was used to clone and express the SNHG6 and KLF6 genes, enabling functional analyses of their roles in chondrosarcoma progression.
Snippet: Human SNHG6 and KLF6 genes were amplified by PCR from cDNA of SW1353 cells with the primers listed in Table 3. SNHG6 and KLF6 genes were then subcloned into the multiple cloning sites (EcoR I/BamH I) of pLenti- P2A-Puro expression plasmid (OriGene, Rockville;induced lncRNA AGAP2-AS1 enhanced the chemoresistance of breast cancer by epigenetic regulation of MyD8818. Jungong Jin and his colleagues identified that SP1-induced lncRNA CASC11 accelerated the tumorigenesis of glioma through targeting FOXK1 via sponging miR-49819. One recent report also revealed that SP1 activation contributed to the high expression of SNHG6 in colorectal cancer cells, which f;- Human SNHG6 and KLF6 genes were amplified by PCR from cDNA of SW1353 cells with the primers listed in Table 3. SNHG6 and KLF6 genes were then subcloned into the multiple cloning sites (EcoR I/BamH I) of pLenti- P2A-Puro expression plasmid (OriGene, Rockville
FigureFigureFigure
Showing 1-6 of 13 papers.
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