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pLenti-P2A-tGFP Lentiviral Gene Expression Vector

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SKU
PS100108
pLenti-P2A-tGFP, tag free Lenti vector with P2A-tGFP, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection tGFP
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF (no stop codon for P2A-tGFP) cloned in this vector will be expressed as native protein, no tag.
  • P2A-tGFP is within the Lentiviral packaging cassette and would allow GFP to enrich both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34 ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Reduced LANCL1-AS1 in old human skeletal muscle diminishes mitochondrial activity, shortens mt-mRNA poly(A) tails, and suppresses myogenesis
Yang, Izydore, Mazan-Mamczarz et al
bioRxiv (2026)
AI Summary: OriGene’s Empty Lentivirus pLenti-P2A-tGFP (Cat# PS100108) was used in this study as a control vector for lentiviral transduction experiments to investigate the functional role of LANCL1-AS1 in skeletal muscle cells.
Snippet: Empty Lentivirus pLenti-P2A-tGFP Origene Cat# PS100108
FigureFigureFigure
Identificación de biomarcadores predictivos de respuesta a inhibidores de la angiogénesis en tumores neuroendocrinos
Lens Pardo
Thesis (2025)
AI Summary: OriGene's pLenti-P2A-tGFP (PS100108) plasmid was utilized in this study to facilitate gene expression and tracking in neuroendocrine tumor cells, contributing to the identification of predictive biomarkers for response to angiogenesis inhibitors.
Snippet: Paz-Ares (CNIO, Spain). pGIPZ-GFP-EV (#120933, Addgene, Watertown, MA, USA), pMD2.G (#12259, Addgene, Watertown, MA, USA) and pLenti-P2A-tGFP (PS100108, OriGene, Rockville, MD, USA) were available in our laboratory. pCD/NLBH*DDD (#17531, Addgene, Watertown, MA, USA) was kindly provided by Dr. Rafael Delgado Vázquez (Instituto
Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury
Yurdagul, Subramanian, Wang et al
Cell Metab. (2020) 31 (3), 518-533.e10 DOI: 10.1016/j.cmet.2020.01.001
Cited by 534
AI Summary: In this study, OriGene's pLenti-P2A-tGFP Lentiviral Particles (Cat# PS100108) and custom Mcf2-pLenti-P2A-tGFP Lentiviral Particles were used to manipulate gene expression in macrophages, enabling the investigation of molecular pathways underlying continual efferocytosis.
Snippet: Skip to article;PE anti-Ly6C Biolegend Cat# 128007;RRID_AB1186133 (1:100 dilution) Bacterial and Virus Strains pLenti-P2A-tGFP Lentiviral Particles Origene Cat# PS100108 Mcf2-pLenti-P2A-tGFP Lentiviral Particles Origene N/A, Custom Request Chemicals, Peptides, and Recombinant Proteins Dulbecco’s Modified Eagle Media (DMEM) Corning Cat# 10-013-CV
Showing 1-3 of 3 papers.
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