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pLenti-C-mCFP-P2A-BSD Lentiviral Gene Expression Vector

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SKU
PS100107
pLenti-C-mCFP-P2A-BSD, Lenti vector with C-terminal mCFP tag and P2A-BSD, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag mCFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Blasticidin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal mCFP tag.
  • P2A-BSD is within the Lentiviral packaging cassette and would allow blasticidin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34 ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mCFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Mcam stabilizes a luminal progenitor-like breast cancer cell state via Ck2 control and Src/Akt/Stat3 attenuation
Balcioglu, Gates, Freeman et al
NPJ Breast Cancer (2024) 10 (1), 80 DOI: 10.1038/s41523-024-00687-7
Cited by 5
AI Summary: OriGene products, including the mouse Mcam shRNA lentiviral plasmid (Cat # TL514377), a scrambled shControl, and a custom shRNA-resistant mouse Mcam construct (pLenti-C-mCFP-P2A-BSD, # PS100107), were used in this study to manipulate Mcam expression and investigate its role in maintaining the luminal progenitor-like state in breast cancer cells.
Snippet: smids were obtained from OriGene Technologies, Inc. (Rockville, MD), including the mouse Mcam shRNA lentiviral plasmid (Cat # TL514377), a scrambled shControl, and a custom shRNA-resistant (via silent-mutation) mouse Mcam construct subcloned into a lentiviral gene expression vector (pLenti-C-mCFP-P2A-BSD), (# PS100107). Comple
FigureFigureFigure
Mcam stabilizes a luminal progenitor-like breast cancer cell state via Ck2 control and Src/Akt/Stat3 attenuation
Balcioglu, Gates, Freeman et al
bioRxiv (2023)
Cited by 2
Snippet: Viral vector plasmids were obtained from OriGene Technologies, Inc. (Rockville, MD), including the mouse Mcam shRNA lentiviral plasmid (Cat # TL514377), a scrambled shControl, and a custom shRNA-resistant (via silent-mutation) mouse Mcam construct subcloned into a lentiviral gene expression vector (pLenti-C-mCFP-P2A-BSD), (# PS100107). Completed vectors were sequenced confirmed
Showing 1-2 of 2 papers.
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