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pLenti-C-HA-IRES-BSD Lentiviral Gene Expression Vector

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SKU
PS100104
pLenti-C-HA-IRES-BSD, Lenti vector with C-terminal HA tag and IRES-BSD, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag HA
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Blasticidin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal HA tag.
  • IRES-BSD is within the Lentiviral packaging cassette and would allow blasticidin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • IRES100 Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia
Grimm, Asuaje, Jain et al
EMBO J (2024)
Cited by 13
AI Summary: OriGene products pLenti-HA-IRES-BSD (Cat# PS100104) and pLenti-GFP-P2A-BSD (Cat# PS100103) were used in this study to manipulate gene expression in human breast cancer cells, enabling the investigation of metabolic enzyme roles in supporting glycolysis, HIF1α stabilization, and cancer cell survival during early hypoxia.
Snippet: pLenti-HA-IRES-BSD Origene Cat# PS100104 pLenti-GFP-P2A-BSD Origene Cat# PS100103 pOTB7-GOT1 Dharmacon Clone ID: BC000498
Tumor-Targeting Nanoparticles for Improved Cancer Therapy
Luo
Thesis (2024)
Snippet: MG5A2039-U) was obtained from Sino Biological (PA, U.S.A). Flag tagged MAPK14 Mouse ORF Clone (Cat.MR227429) was purchased from Origene (MD, USA). LentiCRISPR V2 Plasmid (#52961) was purchased from Addgene (MA, USA). CD44-All-in-one lentiviral sgRNA-CRISPR-Cas9 plasmid was purchased from Horizon
GOT1 primes the cellular response to hypoxia by supporting glycolysis and HIF1α stabilisation
Grimm, Asuaje, Jain et al
bioRxiv (2023)
Snippet: Plasmids pLenti-HA-IRES-BSD (PS100104) and pLenti-GFP-P2A-BSD (PS100103) were from Origene
VRK1 Phosphorylates Tip60/KAT5 and Is Required for H4K16 Acetylation in Response to DNA Damage
García-González, Morejón-García, Campillo-Marcos et al
Cancers (Basel) (2020) 12 (10) DOI: 10.3390/cancers12102986
Cited by 59
AI Summary: OriGene’s pCMV6-mVRK1-myc-DKK plasmid was used to express murine VRK1 in this study, enabling the investigation of VRK1’s role in Tip60 phosphorylation and histone H4K16 acetylation following DNA damage.
Snippet: Murine VRK1 (mVRK1) was expressed from plasmid pCMV6-mVRK1-myc-DKK (OriGene, Rockville, MD, USA). A kinase-dead construct of the murine VRK1(K179E) was generated with the Quick-Mutagenesis system (Stratagene, San Diego, CA, USA) [74]
Showing 1-4 of 4 papers.
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