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pLenti-C-Myc-DDK-P2A-BSD Lentiviral Gene Expression Vector

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SKU
PS100103
pLenti-C-Myc-DDK-P2A-BSD, Lenti vector with C-terminal Myc-DDK tag and P2A-BSD, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Blasticidin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal Myc-DDK tag.
  • P2A-BSD is within the Lentiviral packaging cassette and would allow blasticidin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34 ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • DDK-Myc_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

The molecular and functional landscape of resistance to FOLFIRI chemotherapy in metastatic colorectal cancer
Avolio, Leto, Sassi et al
Cancer Discov (2025)
AI Summary: OriGene products contributed to this study by providing the pLenti-C-Myc-DDK-P2A-Puro (PS100092) and pLenti-C-Myc-DDK-P2A-BSD (PS100103) lentiviral vectors used for overexpressing human RAD51 and RING1 cDNAs, respectively, enabling functional analysis of these genes in chemotherapy resistance models.
Snippet: The cDNA of human RAD51 (NM_002875) was cloned into the pLenti-C-Myc-DDK-P2A-Puro lentiviral vector (PS100092, OriGene). The cDNA of human RING1 (NM_002931) was cloned into the pLenti-CMyc-DDK-P2A-BSD lentiviral vector (PS100103, OriGene). …
Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia
Grimm, Asuaje, Jain et al
EMBO J (2024)
Cited by 13
AI Summary: OriGene products pLenti-HA-IRES-BSD (Cat# PS100104) and pLenti-GFP-P2A-BSD (Cat# PS100103) were used in this study to manipulate gene expression in human breast cancer cells, enabling the investigation of metabolic enzyme roles in supporting glycolysis, HIF1α stabilization, and cancer cell survival during early hypoxia.
Snippet: pLenti-HA-IRES-BSD Origene Cat# PS100104 pLenti-GFP-P2A-BSD Origene Cat# PS100103 pOTB7-GOT1 Dharmacon Clone ID: BC000498
Tumor-Targeting Nanoparticles for Improved Cancer Therapy
Luo
Thesis (2024)
Snippet: MG5A2039-U) was obtained from Sino Biological (PA, U.S.A). Flag tagged MAPK14 Mouse ORF Clone (Cat.MR227429) was purchased from Origene (MD, USA). LentiCRISPR V2 Plasmid (#52961) was purchased from Addgene (MA, USA). CD44-All-in-one lentiviral sgRNA-CRISPR-Cas9 plasmid was purchased from Horizon
GOT1 primes the cellular response to hypoxia by supporting glycolysis and HIF1α stabilisation
Grimm, Asuaje, Jain et al
bioRxiv (2023)
Snippet: Plasmids pLenti-HA-IRES-BSD (PS100104) and pLenti-GFP-P2A-BSD (PS100103) were from Origene
Caspases Switch off the m6A RNA Modification Pathway to Foster the Replication of a Ubiquitous Human Tumor Virus
Zhang, Zhang, Maharjan et al
mBio (2021) 12 (4), e0170621 DOI: 10.1128/mBio.01706-21
AI Summary: OriGene products pLenti-C-Myc-DDK-P2A-BSD and pCMV6-Entry-YTHDF2 were used for lentiviral transduction and expression of YTHDF2 variants, enabling the study of YTHDF2’s role in regulating m6A RNA modification and Epstein-Barr virus replication.
Snippet: 10mg/ml blasticidin. The target guide RNA sequences are listed in Table S1A. Lentiviral transduction of YTHDF2. The pLenti-C-Myc-DDK-P2A-BSD and pCMV6-Entry-YTHDF2 were purchased from Origene. The specific variants
Showing 1-5 of 5 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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