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pLenti-mRFP-P2A-Puro Lentiviral Gene Expression Vector

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SKU
PS100094
pLenti-C-mRFP-P2A-Puro, Lenti vector with C-terminal mRFP tag and P2A-Puro, 10 ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag mRFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric RFP tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • RFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

p53 gain-of-function mutation induces metastasis via Brd4-dependent Csf-1 expression
Efe, Dunbar, Sugiura et al
Cancer Discov (2023)
AI Summary: OriGene products contributed to this study by providing the Csf-1 (Myc-DDK-tagged) cDNA (MR226827) and the pLenti-C432 mRFP-P2A-Puro-Lenti vector (PS100094), which were used for cloning and functional analysis of Csf-1 in the context of p53-induced metastasis.
Snippet: The Csf-1 (Myc-DDK-tagged) cDNA (Origene, MR226827) was cloned into the pLenti-C432 mRFP-P2A-Puro-Lenti vector (Origene, PS100094) at the SgfI-MluI restriction enzyme sites
Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation
Chen, Chang, Hsu et al
Nat Commun (2022) 13 (1), 2945 DOI: 10.1038/s41467-022-30638-4
Cited by 39
AI Summary: OriGene’s pLenti-C-mGFP-P2A-Puro and pLenti-C-mRFP-P2A-Puro vectors were used to construct fluorescently tagged ATP1A1 expression plasmids, enabling the study of ATP1A1-mediated cell-cell interactions in tumor and fibroblast cells in this research.
Snippet: …Pancreatic ductal Save;pLenti-ATP1A1-GFP and pLenti-ATP1A1-RFP were constructed by insertion of cDNA of ATP1A1 (NM_000701.7) at Sgf1 site and MluI site of pLenti-C-mGFP-P2A-Puro (Origene) and pLenti-C-mRFP-P2A-Puro (Origene) vectors
Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity
Lazzari, Nicolas, Matsusaki et al
Acta Biomater (2018)
AI Summary: In this study, OriGene's pLenti-C-mGFP-P2A-Puro particles were used to transduce MRC-5 fibroblasts, enabling fluorescent labeling for tracking within the novel 3D pancreatic tumor spheroid model.
Snippet: GFP)-transduction, MRC-5 fibroblasts (9 x 10 4 cells.mL -1 ) were seeded in 24-well plates (1 mL/well) and incubated for 24 h in a humid atmosphere at 37 °C with 5% CO 2 . Transduction was performed by addition of pLenti-C-mGFP-P2A-Puro particles (Origene
Showing 1-3 of 3 papers.
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