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pLenti-C-Myc-DDK-P2A-tGFP Lentiviral Gene Expression Vector

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SKU
PS100088
pLenti-C-Myc-DDK-P2A-tGFP, Lenti vector with C-terminal Myc-DDK tag and tGFP after P2A peptide, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection tGFP
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • P2A-tGFP is within the Lentiviral packaging cassette and would allow tGFP expression in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Endothelial STING and STAT1 mediate interferon-independent effects of IL-6 in an endotoxemia-induced model of shock
Martino, Sanders, Bossardi Ramos et al
J Clin Invest (2025)
AI Summary: OriGene products contributed to this study by providing the Human Tagged Lenti ORF Clone for IRF1 (catalog number RC203500L1) and the pLenti-C-Myc-DDK-P2A-tGFP lentiviral gene expression vector (PS100088), which were used to generate lentiviral constructs for IRF1 expression in functional experiments.
Snippet: Lentiviral constructs to express IRF1 with C-terminal 577 Myc and DDK tags were generated by cloning IRF1 from a Human Tagged Lenti ORF Clone 578 (Origene catalog number RC203500L1) into a pLenti-C-Myc-DDK-P2A-tGFP (Origene 579 PS100088) lentiviral gene expression vector
Showing 1-1 of 1 papers.
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