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pLenti-EF1a-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

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SKU
PS100085
pLenti-EF1a-C-Myc-DDK-IRES-Puro, Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter EF1a
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • IRES100 Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Characterization of the Secretome of a Specific Cell Expressing Mutant Methionyl-tRNA Synthetase in Co-Culture Using Click Chemistry
Shin, Lee, Choi et al
Int J Mol Sci (2022) 23 (12) DOI: 10.3390/ijms23126527
Cited by 17
AI Summary: OriGene products contributed to this study by providing the TrueORF cDNA clone (Cat# MR225771) used for the expression of mouse mutant methionyl-tRNA synthetase, enabling cell-specific labeling and analysis of the secretome in co-culture experiments.
FigureFigureFigure
Shigella OspC3 suppresses murine cytosolic LPS sensing
Oh, Verma, Hafeez et al
iScience (2021) 24 (8), 102910 DOI: 10.1016/j.isci.2021.102910
Cited by 38
AI Summary: OriGene’s pLenti-EF1a-C-Myc-DDK-IRES-Puro plasmid (Cat# PS100085) was used as a recombinant DNA backbone for constructing expression vectors in this study investigating how Shigella OspC3 suppresses cytosolic LPS sensing.
Snippet: aboratory JAX# 021614 Mouse: 129/SvEv: 129S1/SvImJ The Jackson Laboratory JAX# 002448 ------------------------- Recombinant DNA ------------------------- pLenti-EF1a-C-Myc-DDK-IRES-Puro OriGene Cat# PS100085 pLenti-EF1a-C-Myc-DDK-IRES-Puro-Casp-1 This paper N/A pLenti-EF1a-C-Myc-DDK-IRES-Puro-Casp-4 This paper N/A pLenti-EF1a-C-Myc-DDK-IRES-Puro-Casp-5 This paper
FigureFigureFigure
Combined 4-1BB and ICOS co-stimulation improves anti-tumor efficacy and persistence of dual anti-CD19/CD20 chimeric antigen receptor T cells
Wang, Zhong, Ke et al
Cytotherapy (2021) 23 (8), 715-723 DOI: 10.1016/j.jcyt.2021.02.117
Cited by 22
AI Summary: OriGene’s lentiviral transfer plasmid pAC001 (derived from PS100085) was used to clone the novel CAR constructs, enabling efficient gene delivery and expression in the development and testing of dual anti-CD19/CD20 CAR T cells in this study.
Snippet: CD20 scFvs were designed (Figure 1A) and synthesized, followed by cloning into a lentiviral transfer plasmid, pAC001, a self-inactivating lentiviral vector, derived from PS100085 (OriGene). CAR gene expression was driven by human elongation factor promoter EF-1a. …
A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy
Ni, Afroze, Xing et al
Genet Med (2021)
Cited by 37
AI Summary: OriGene’s pLenti-EF1a-C-Myc-DDK-IRES-Puro vector was used to clone UFSP2 gene fragments for reconstitution experiments in patient-derived fibroblasts, enabling the study of the variant’s functional effects in this paper.
Snippet: … We identified a homozygous variant in the gene encoding UFM1-specific peptidase 2 (UFSP2), which participates in the UFMylation pathway of protein modification. UFSP2 variants are implicated;The fragments were purified and cloned into pLenti-EF1a-C-Myc-DDK-IRES-Puro (OriGene) using the In-Fusion HD Cloning Kit following the manufacturer’s instructions (Takara Bio)
Bromodomain-containing protein 4 regulates interleukin-34 expression in mouse ovarian cancer cells
Han, Anwar, Hama et al
Inflamm Regen (2020) 40, 25 DOI: 10.1186/s41232-020-00129-4
Cited by 9
AI Summary: In this study, the mouse Il34 cDNA was cloned into the OriGene pLenti-EF1a-C-Myc-DDK-IRES-Puro lentiviral vector to generate lentiviral constructs for investigating the regulation of IL-34 expression in ovarian cancer cells.
Snippet: primers as follows: forward 5′-ATGCCCTGGGGACTCGCCTG-3′ and reverse 5′-TCAGGGCAACGAGCCATGGC-3′. The obtained moue Il34 cDNA was cloned into lentiviral vector, pLenti-EF1a-C-Myc-DDK-IRES-Puro (OriGene, MD, USA). Lenti-X293T cells (Takara bio, Shiga, Japan) were transfected with the lentiviral vector and two packaging plasmids pCMV-VSV-G-RSV-Rev (RIKEN BRC DNA BANK, Tsukuba, Japan) and pCAG
FigureFigureFigure
Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival
Otsuka, Wada, Tsuji et al
Sci Rep (2020) 10 (1), 224 DOI: 10.1038/s41598-019-57088-1
Cited by 39
AI Summary: OriGene’s pLenti-EF1a-C-Myc-DDK-IRES-Puro vector was used to clone and express the mouse Foxn1 gene in induced pluripotent stem cells, enabling the efficient generation of thymic epithelial-like tissue described in the study.
Snippet: boratory). Cells were maintained in a 5% CO2/air environment at 37 °C. Cloning and gene transduction with lentiviral vector Mouse Foxn1 CDS was cloned into pLenti-EF1a-C-Myc-DDK-IRES-Puro vector (Origene), and B6 iPSCs were infected with lentivirus. Lenti-X293T cells were transfected with lentiviral vector and two packaging plasmids, pMD2.G and psPAX2 (Addgene), using polyethylene imine. Lenti
FigureFigureFigure
Showing 1-6 of 13 papers.
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