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pLenti-EF1a-C-mGFP Lentiviral Gene Expression Vector

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SKU
PS100084
pLenti-EF1a-C-mGFP, Lenti Vector with EF1 alpha promoter and C-terminal mGFP tag, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Expression Promoter EF1a
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal mGFP tag.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • A brief lentiviral packaging protocol is provided.
    • E. Coli selection: Chloramphenicol(34ug/ml)
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

11q Deletion or ALK Activity Curbs DLG2 Expression to Maintain an Undifferentiated State in Neuroblastoma
Siaw, Javanmardi, Van den Eynden et al
Cell Rep (2020) 32 (12), 108171 DOI: 10.1016/j.celrep.2020.108171
Cited by 54
AI Summary: OriGene products, specifically pLenti-DLG2-mGFP and pLenti-mGFP lentiviral particles, were used to manipulate DLG2 expression in neuroblastoma cell lines, enabling the authors to study the role of DLG2 in cell differentiation.
Snippet: pLenti-DLG2-mGFP and pLenti-mGFP lentiviral particles were purchased from Origene Technologies. … SMARTvector inducible non-targeting control, shCtrl, DLG2 shRNA;shDLG2 #1and shDLG2 #2 lentiviral particles were purchased from Horizon and described in the Key Resources Table. SP1(NM_138473.3) ORF cloned into pcDNA3.1 by, and ordered from, Genscript. …
Myo1f, an Unconventional Long-Tailed Myosin, Is a New Partner for the Adaptor 3BP2 Involved in Mast Cell Migration
Navinés-Ferrer, Ainsua-Enrich, Serrano-Candelas et al
Front Immunol (2019) 10, 1058 DOI: 10.3389/fimmu.2019.01058
AI Summary: OriGene’s flag-tagged Myo1f construct (cloned in pCMV6-Entry plasmid) was used to study the interaction between Myo1f and 3BP2 and their roles in mast cell migration.
Snippet: Cells were transfected with a flag-tagged Myo1f construct from (OriGene Technologies, Rockville, MD), cloned into a pCMV6-Entry plasmid. 3BP2 cloned in pCDNA 3.1 and Fyn cloned in psrα used in COS transfections were described in Saborit et al Cached
FigureFigureFigure
Empowering human cardiac progenitor cells by P2Y14 nucleotide receptor overexpression
Khalafalla, Kayani, Kassab et al
J. Physiol. (Lond.) (2017)
AI Summary: OriGene contributed to this study by providing the lentiviral plasmids encoding P2Y14R fused to mGFP and monomeric GFP (mGFP), which were used to overexpress P2Y14 receptor in human cardiac progenitor cells and assess its effects on cell function.
Snippet: in a 6-well plate (30,000 cells/well) overnight then transduced with lentiviral particles encoding monomeric green fluorescent protein (2 MOI;hCPC-mGFP) or P2Y14R fused to mGFP (0.2 MOI;hCPC-Y14) (lentiviral plasmids were purchased from Origene - P2Y14R ...;... in a 6-well plate (30,000 cells/well) overnight then transduced with lentiviral particles encoding monomeric green fluorescent protein (2 ...
The Adaptor 3BP2 Is Required for KIT Receptor Expression and Human Mast Cell Survival
Ainsua-Enrich, Serrano-Candelas, Álvarez-Errico et al
J. Immunol. (2015)
Cited by 22
AI Summary: OriGene products, specifically mGFP-tagged and Lenti vector constructs, were used for 3BP2 and MITF reconstitution experiments to assess their roles in KIT expression and human mast cell survival in this study.
Snippet: mGFP tagged, and Lenti vector with C-terminal monomeric GFP tag as a control (OriGene Technologies, Rockville, MD) were used for 3BP2 and MITF reconstitution experiments, respectively. Lentiviral particles expressing miRNA ... Cite SaveSaving
Showing 1-4 of 4 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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