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pLenti-C-Myc-DDK-IRES-Neo Lentiviral Gene Expression Vector

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SKU
PS100081
pLenti-C-Myc-DDK-IRES-Neo, Lenti vector with bicistronic IRES-Neo, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Neo is within the Lentiviral packaging cassette and would allow Neomycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
    • E. Coli selection: Chloramphenicol(34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • WPRE_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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NONO, SFPQ, and PSPC1 promote telomerase recruitment to the telomere
Sobinoff, Wells, Chow et al
Nat Commun (2025) 16 (1), 5769 DOI: 10.1038/s41467-025-60924-w
Cited by 7
AI Summary: OriGene products, specifically the pLenti-C-Myc-DDK-IRES-Puro (PS100069) and pLenti-C-Myc-DDK-IRES-Neo (PS100081) plasmid backbones, were used for cloning wild-type and mutant DBHS proteins to study their role in telomerase recruitment to telomeres.
Snippet: nd hTR stability (3). MethodsPlasmids Wild type DBHS proteins (NONO, SFPQ, PSPC1) and their functional mutants (ΔRRM1, ΔRRM2, ΔPol, ΔDim) were cloned into the pLenti-C-Myc-DDK-IRES-Puro backbone (Origene Technologies;PS100069) and/or the pLenti-C-Myc-DDK-IRES-Neo backbone (Origene Technologies;PS100081) by restriction enzyme subcloning of gene blocks synthesized by Integrated DNA Technologie
FigureFigureFigure
High cereblon expression in neuroendocrine cancer confers vulnerability to GSPT1 molecular glue degrader
Park, Joo, Kim et al
Exp Hematol Oncol (2025) 14 (1), 89 DOI: 10.1186/s40164-025-00674-z
Cited by 3
AI Summary: OriGene’s pLenti-C-Myc-DDK-IRES-Neo lentiviral vector (#PS100081) was used to generate CRBN-overexpressing cell lines, enabling the study to demonstrate how increased CRBN expression enhances neuroendocrine cancer cell sensitivity to GSPT1 molecular glue degraders.
Snippet: n for further assays. To establish a CRBN-overexpressing cell line, the full-length cDNA of CRBN was cloned from the plasmid pcDNA3.1-hCRBN [29] into the pLenti-C-Myc-DDK-IRES-Neo lentiviral vector (Origene, #PS100081). Lentivirus production was performed by transfecting the CRBN-expressing vector or an empty control vector into Lenti-X 293 T cells (Takara, #632,180) along with the psPAX2 and pM
FigureFigureFigure
Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies
Voon, Hii, Garvie et al
Genome Biol (2023) 24 (1), 284 DOI: 10.1186/s13059-023-03122-5
Cited by 20
AI Summary: OriGene’s pLenti-C-Myc-DDK-IRES-Neo expression vector (catalog #PS100081) was used to generate a PML isoform 1 expression construct for functional studies in this paper.
Snippet: DDK-IRES-Neo, Genscript) cassette was used in place of dCas9-KRAB. To generate PML I expression construct, PML isoform 1 ORF (Genscript) was cloned into a pLenti-C-Myc-DDK-IRES-Neo expression vector (Origene #PS100081). Lentiviral particles were produced in human HEK293 cells as described above. Briefly, HEK293 cells were transiently transfected with pLenti-C-Myc-DDK-IRES-Neo plasmid and packaging
FigureFigureFigure
Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancers
Mao, Zhang, Lee et al
Nat Commun (2021) 12 (1), 5053 DOI: 10.1038/s41467-021-25341-9
Cited by 31
AI Summary: OriGene’s pLenti-Lentiviral vector (pLenti-C-Myc-DDK-P2A-Neo, PS100081) was used to achieve stable gene expression in cell models, enabling the authors to investigate PI3K pathway dependencies in prostate cancer subtypes.
Snippet: the reaction. All cell proliferation assays were repeated in at least two independent biological replicates. Stable gene expression analysis. pLenti-Lentiviral vector (pLenti-C-Myc-DDK-P2A-Neo) (Origene, PS100081) was used
AKT1 E17K Inhibits Cancer Cell Migration by Abrogating β-Catenin Signaling
Gao, Kiliti, Zhang et al
Mol Cancer Res (2020)
AI Summary: In this study, OriGene's PIK3CA E545K mutant cDNA (Cat# RC400348) and Lenti-C-Myc-DDK-IRES-Neo plasmid (Cat# PS100081) were used to generate a PIK3CA-E545K lentiviral expression vector for functional analyses of cancer cell migration.
Snippet: … PIK3CA E545K mutant cDNA (Cat#RC400348), purchased from OriGene, was inserted into the AsiS I and Mlu I sites of plasmid Lenti-C-Myc-DDK- IRES-Neo (Cat#PS100081, OriGene), to generate PIK3CA-E545K lentiviral expression vector;- cloning purpose. PIK3CA E545K mutant cDNA (Cat#RC400348), purchased from OriGene, was inserted into the AsiS I and Mlu I sites of plasmid Lenti-C-Myc-DDK- IRES-Neo (Cat#PS100081, OriGene), to generate PIK3CA-E545K lentiviral expression vector
HILPDA regulates lipid metabolism, lipid droplet abundance and response to microenvironmental stress in solid tumors
VandeKopple, Wu, Auer et al
Mol. Cancer Res. (2019)
AI Summary: In this study, OriGene's rabbit anti-Perilipin2 antibody (TA321279) was used to detect Perilipin2 protein, aiding the investigation of lipid droplet abundance and lipid metabolism regulated by HILPDA in tumor cells.
Snippet: Primary antibodies used were: custom-made rabbit anti-Hilpda 1:250 (29), rabbit a- Perilipin2 (1:1000, Origene TA321279), rabbit a-SCD1 (Cell Signaling 2438), rabbit a-G0S2 (Epigentek A50229), rabbit a-ELOVL6 (Millipore Sigma ABS458), rabbit a-LC3 (1:1000
WB
Showing 1-6 of 13 papers.
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