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pLenti-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

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SKU
PS100069
pLenti-C-Myc-DDK-IRES-Puro, Lenti vector with bicistronic IRES-Puro, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags
    • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • IRES100 Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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Contrasting impacts of two ABCB11 variants affecting the same residue in progressive familial intrahepatic cholestasis type 2
Riahi, Almes, Banet et al
Am J Physiol Gastrointest Liver Physiol (2026) 331 (3), G299-G311 DOI: 10.1152/ajpgi.00055.2026
AI Summary: OriGene’s pLenti-cMyc-DDK-IRES-Puro plasmid (PS100069) was used as a vector backbone for cloning the human NTCP-encoding cDNA, enabling functional studies of bile acid transport relevant to the characterization of ABCB11 variants in PFIC2.
Snippet: a basolateral BA transporter, is required (26). The human NTCP-encoding cDNA was cloned into the lentiviral vector pLenti-cMyc-DDK-IRES-Puro plasmid (PS100069;OriGene, Rockville, MD) in which the cytomegalovirus (CMV) promoter was replaced by a human phosphoglycerate kinase (PGK) promoter (Supplemental Fig. S2)
METTL3 promotes cell-cycle progression via activation of transcriptional elongation
Akçaöz-Alasar, Wang, Li et al
Mol Cell (2026) 86 (16), 3213-3222.e5 DOI: 10.1016/j.molcel.2026.07.011
AI Summary: In this study, the OriGene product pLenti-C-Myc-DDK-IRES-Puro was used to facilitate gene expression experiments investigating the role of METTL3 in cell-cycle progression and transcriptional elongation.
Snippet: pCMV-VSVG Addgene 138479 pCMV-dR.8 Addgene 8455 pLenti-C-Myc-DDK-IRES-Puro Origene PS100069
Targeting SLC1A5-mediated glutamine metabolism overcomes PARP inhibitor resistance in ovarian cancer
Han, Du, Tian et al
Cell Death and Disease (2026)
AI Summary: OriGene's pCMV vector (PS100069) was used to generate lentiviral overexpression constructs for USP14 and SLC1A5, enabling the study of their roles in glutamine metabolism and PARP inhibitor resistance in ovarian cancer.
Snippet: The lentiviral overexpression constructs for USP14 and SLC1A5 were generated by cloning the open reading frames (ORFs) of human USP14 and SLC1A5 into the pCMV vector (PS100069, OriGene, Rockville, MD, USA)
Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer
Setayeshpour, Chen, Dayanidhi et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-72116-1
Cited by 4
AI Summary: OriGene products contributed to this study by providing the mouse HMGCS2 ORF clone in the pCMV6-Entry backbone (MG208162 and RC208128) for overexpression experiments and the lentiviral vector (PS100069) for generating stable cell lines, enabling functional analyses of HMGCS2 in ovarian cancer ferroptosis resistance.
Snippet: … For transient overexpression of HMGCS2, 100 ng of mouse _hmgcs2_ ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the TransIT -LT1 transfection reagent (Mirus, MIR2305) was added before the mix was incubated for 15 min in a 96-well plate;plasmids was conducted using the Qiagen Plasmid Midi kit (12145). For transient overexpression of HMGCS2, 100 ng of mouse hmgcs2 ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the;- HMGCS2 ORF clone with a pCMV6-Entry backbone (Origene, RC208128) was cut and ligated into a lentiviral vector (Origene, PS100069) according to the manufacturer’s
FigureFigureFigure
Perilipin 5 enhances glycogen storage and mitigates excessive fatty acid oxidation in skeletal muscle to alleviate mitochondrial impairment
Xu, Gao, Xu et al
Biochim Biophys Acta Mol Cell Res (2026) 1873 (5), 120146 DOI: 10.1016/j.bbamcr.2026.120146
AI Summary: OriGene’s pLenti-CMV-Empty plasmid (ps100069) was used as a control in lentiviral transfection experiments to study the effects of Plin5 overexpression in 293T cells.
Snippet: , the 293 T cells were co-transfected with lentivirus vector plasmid (pLenti-CMV-Empty [Origene, ps100069], pLenti-CMV-mPlin5) and auxiliary packaging plasmid (pAX2 and pVSVG)
NONO, SFPQ, and PSPC1 promote telomerase recruitment to the telomere
Sobinoff, Wells, Chow et al
Nat Commun (2025) 16 (1), 5769 DOI: 10.1038/s41467-025-60924-w
Cited by 7
AI Summary: OriGene products, specifically the pLenti-C-Myc-DDK-IRES-Puro (PS100069) and pLenti-C-Myc-DDK-IRES-Neo (PS100081) plasmid backbones, were used for cloning wild-type and mutant DBHS proteins to study their role in telomerase recruitment to telomeres.
Snippet: nd hTR stability (3). MethodsPlasmids Wild type DBHS proteins (NONO, SFPQ, PSPC1) and their functional mutants (ΔRRM1, ΔRRM2, ΔPol, ΔDim) were cloned into the pLenti-C-Myc-DDK-IRES-Puro backbone (Origene Technologies;PS100069) and/or the pLenti-C-Myc-DDK-IRES-Neo backbone (Origene Technologies;PS100081) by restriction enzyme subcloning of gene blocks synthesized by Integrated DNA Technologie
FigureFigureFigure
Showing 1-6 of 37 papers.
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