OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

pEX-1 Bacterial Expression Vector

Be the first to review this product
SKU
PS100075
pEX-1, bacterial expression vector with no tags, 10ug
  $470.00
In Stock*

Frequently Bought Together (2)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00
Specifications
Specifications
Product Data
E. coli Selection Ampicillin (100 ug/mL)
Features

This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as untagged proteins.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vectors.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping Blue Ice
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Mig-6 Inhibits Autophagy in HCC Cell Lines by Modulating miR-193a-3p
Qu, Tian, Hong et al
Int J Med Sci (2022) 19 (2), 338-351 DOI: 10.7150/ijms.66040
Cited by 15
AI Summary: In this study, OriGene’s human Mig-6 overexpression plasmid (RC209726) and Mig-6 shRNA plasmids (TG311478, TG311479) were used to manipulate Mig-6 expression in HCC cell lines, enabling the authors to investigate the role of Mig-6 in regulating apoptosis and autophagy via miR-193a-3p.
FigureFigureFigure
TGFBR3L is an inhibin B co-receptor that regulates female fertility
Brûlé, Wang, Li et al
Sci Adv (2021) 7 (51), eabl4391 DOI: 10.1126/sciadv.abl4391
Cited by 40
AI Summary: OriGene’s human TGFBR3L expression vector was used to express TGFBR3L in heterologous cells, enabling the authors to study its role as an inhibin B co-receptor in female fertility.
Snippet: When we expressed human TGFBR3L in heterologous cells using a commercially sourced expression vector (OriGene), the protein Revised human TGFBR3L-Myc-DDK was made in-house by excising the 5′ end of the coding sequence from the OriGene Save;When we expressed human TGFBR3L in heterologous cells using a commercially sourced expression vector (OriGene),
FigureFigureFigure
Warfarin and vitamin K compete for binding to Phe55 in human VKOR
Czogalla, Biswas, Höning et al
Nat. Struct. Mol. Biol. (2016)
AI Summary: OriGene’s F9 expression vector (cat. no. SC126517) was used to express F9 in the study, enabling functional assays to investigate how specific VKOR mutations affect vitamin K and warfarin binding.
Snippet: For critical residues suspected to influence vitamin K binding, site-directed mutagenesis was performed. For expression of only F9, an expression vector from Origene was used (cat. no. SC 126517). Human NQO1 and VKORC1L1 cDNAs were independently cloned together with F9 cDNA into the pIRES vector
A common genetic variation of melanoma inhibitory activity-2 labels a subtype of pancreatic adenocarcinoma with high endoplasmic reticulum stress levels
Kong, Wu, Valkovska et al
Sci Rep (2015) 5, 8109 DOI: 10.1038/srep08109
Cited by 20
AI Summary: OriGene’s HNF1A expression vector (RC211201) was used to transiently express HNF1A in pancreatic cancer cells, enabling the study of its role in regulating MIA2 expression and ER stress in this research on pancreatic adenocarcinoma subtypes.
Snippet: … Su86.86 cells were transfected with different MIA2 expressing vectors using the LipofectamineH 2000 transfection reagent (Invitrogen, Carlsbad, California) according to the manufacturer’s instructions. For transient expression of HNF1A, the expression vector from Origene was used (RC211201, Origene, Suite 200 Rockville, MD). …
FigureFigureFigure
Canavanine induces insulin release via activation of imidazoline I-3 receptors
Yang, Niu, Chen et al
Clin. Exp. Pharmacol. Physiol. (2014)
Cited by 8
AI Summary: OriGene products contributed to this study by providing the human nischarin (NISCH) expression vector and specific antibodies for NISCH and DDK, which were used to create and characterize transfected CHO-K1 cells expressing imidazoline receptors for investigating canavanine’s effects.
Snippet: human nischarin (NISCH), a mouse homologue of the human imidazoline receptor antisera-selective (IRAS) protein, and an expression vector (Origene, Rockville, MD, USA) ... specific for NISCH and DDK (Origene, Rockville, MD, USA) as described previously (19). ... Cite SaveSaving
Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen
Wu, Pfeiffer, Calhoon et al
J. Biol. Chem. (2011) 286 (28), 24916-30 DOI: 10.1074/jbc.M111.247163
Cited by 2
AI Summary: OriGene's anti-DDK antibodies were used in this study for Western blot analyses to detect and confirm the expression of TMEM86b protein following its overexpression and cloning.
Snippet: Plasmalogen levels are decreased in the peroxisomal disorders (5), Alzheimer disease (6), and Down syndrome (7), and they are elevated in tumors and in cell lines derived from tumors (8). Moreover, the ... 9126), and antibodies against DDK were from OriGene Technologies, Inc
FigureFigureFigure
WB
Showing 1-6 of 7 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products