Fli1 Rat shRNA Plasmid (Locus ID 315532)

SKU
TR702443
Fli1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
2 Weeks*
Specifications
Specifications
Product Data
Locus ID 315532
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Fli1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 315532). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_001017381, NM_001017381.1
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Targeting the CALR interactome in myeloproliferative neoplasms
Pronier, Cifani, Merlinsky et al
JCI Insight (2018) 3 (22) DOI: 10.1172/jci.insight.122703
AI Summary: OriGene’s shRNA-Erp57 and shRNA-Fli1 products were used to knock down ERp57 and FLI1 expression in Ba/F3 cells, enabling the study to investigate the functional roles of these CALR-interacting proteins in myeloproliferative neoplasms.
Snippet: –505 bp. Fli1 and Erp57 knock down by retroviral delivery of shRNA. Ba/F3 cells were transduced as previously described (30) with retroviruses expressing puromycin and shRNA-_Erp57_, shRNA-_Fli1_ (OriGene), or shRNA-_Renilla_ as control. Cells were infected twice in IL-3–containing media, followed by puromycin selection (2 μg/ml, 3–6 days). Fli1 ChIP-seq. Briefly, MPL-WT-Ba/F3 cells were
Showing 1-1 of 1 papers.
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