OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Hif1a Mouse shRNA Plasmid (Locus ID 15251)

Be the first to review this product
SKU
TR517255
Hif1a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
In Stock*

Frequently Bought Together (1)

TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Hif1a or NM_010431"

Specifications
Specifications
Product Data
Locus ID 15251
Synonyms AA959795; bHLHe7; bHLHe78; HIF-1; HIF-1-alpha; HIF1; HIF1-alpha; HIF1alpha; MO; MOP1
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Hif1a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 15251). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC026139, NM_010431, NM_010431.1, NM_010431.2
UniProt ID Q61221
Summary This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2015
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Secretion-mediated STAT3 activation promotes self-renewal of glioma stem-like cells during hypoxia
Almiron Bonnin, Havrda, Lee et al
Oncogene (2017)
Cited by 94
AI Summary: In this study, OriGene's HuSH shRNA constructs were used to specifically reduce mouse HIF-1α gene expression, enabling the authors to investigate the role of HIF-1α in hypoxia-induced self-renewal of glioma stem-like cells.
Snippet: Original Article.;HuSHTM shRNA constructs (Origene Technologies, Rockville, MD, USA) were used to reduce gene expression of mouse HIF-1 α . MISSION shRNA constructs (Sigma-Aldrich) were used to reduce the expression of JAK1 and JAK2. shRNA sequences are provided in Supplementary Table 4
Role of hypoxia-inducible factors in the dexrazoxane-mediated protection of cardiomyocytes from doxorubicin-induced toxicity
Spagnuolo, Recalcati, Tacchini et al
Br. J. Pharmacol. (2011) 163 (2), 299-312 DOI: 10.1111/j.1476-5381.2011.01208.x
Cited by 41
AI Summary: In this study, OriGene’s HIF-1α-specific shRNA and non-effective shGFP sequence cassette plasmids were used to genetically manipulate HIF levels in cardiomyocytes, enabling the researchers to demonstrate that HIF activation is a key mechanism in dexrazoxane-mediated protection against doxorubicin-induced toxicity.
Snippet: 600 ng each) containing the HIF-1α-specific shRNA, or with plasmids containing a non-effective shGFP sequence cassette (Origene Technologies, Inc ... 2 h, formazan crystals were dissolved by adding 500 µL of the MTT solubilization solution and thorough up-and-down pipetting
Adenosine A(2)A receptor but not HIF-1 mediates Tyrosine hydroxylase induction in hypoxic PC12 cells
Gammella, Cairo, Tacchini
J Neurosci Res (2010) 88 (9), 2007-16 DOI: 10.1002/jnr.22366
Cited by 9
AI Summary: In this study, OriGene’s pCMV6-XL5-TH expression plasmid was used to investigate the regulation of tyrosine hydroxylase (TH) expression in PC12 cells under hypoxic conditions, helping to demonstrate that TH induction is mediated by adenosine A(2)A receptor signaling rather than HIF-1.
Role of HIF-1 and NF-kappaB transcription factors in the modulation of transferrin receptor by inflammatory and anti-inflammatory signals
Tacchini, Gammella, De Ponti et al
J Biol Chem (2008) 283 (30), 20674-86 DOI: 10.1074/jbc.M800365200
AI Summary: OriGene products contributed to this paper by providing specific siRNA reagents (OriGene, SR415014 for HIF-1α and SR415016 for HIF-1β) used to silence HIF-1 expression in macrophages, enabling the authors to investigate the role of HIF-1 in regulating transferrin receptor (TfR1) expression under inflammatory and anti-inflammatory conditions.
ROLE OF HIF ACTIVATION IN THE PROTECTION OF CARDIOMYOCYTES FROM DOXORUBICIN TOXICITY
SPAGNUOLO
Thesis (2012)
Snippet: Short hairpin RNA (shRNA) constructs against Mus musculus HIF-1a (catalogue number TR517255) were purchased from Origene Technologies, Inc. (Rockville, MD, USA). The targeted sequences were:
Showing 1-6 of 6 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products