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ATG5 Human shRNA Plasmid Kit (Locus ID 9474)

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SKU
TR314610
ATG5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 9474
Synonyms APG5; APG5-LIKE; APG5L; ASP; hAPG5; SCAR25
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components ATG5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9474). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_001286106, NM_001286107, NM_001286108, NM_001286111, NM_004849, NR_104402, NR_104403, NM_004849.1, NM_004849.2, NM_004849.3, NM_001286111.1, NM_001286107.1, NM_001286108.1, NM_001286106.1, BC093011, BC093011.1, BC002699, BM831472, NM_004849.4
UniProt ID Q9H1Y0
Summary The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. provided by RefSeq, Sep 2015
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
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file-alt Citations

ATGs ubiquitination is required for circumsporozoite protein to subvert host innate immunity against malaria liver stage
Zheng, Lu, Li et al
bioRxiv (2021)
Cited by 2
Snippet: RNA interference for ATG5 and ATG7 in HepG2 cells was performed according to the protocol of ATG5/7 Human shRNA Plasmid Kits (Origene, Rockville, MD, USA)
An analog of glibenclamide selectively enhances autophagic degradation of misfolded α1-antitrypsin Z
Wang, Cobanoglu, Li et al
PLoS ONE (2019) 14 (1), e0209748 DOI: 10.1371/journal.pone.0209748
AI Summary: OriGene products contributed to this study by providing shRNA constructs used to generate ATG5 knockdown cell lines (HTOZATG5KD), enabling investigation of autophagy’s role in the degradation of misfolded α1-antitrypsin Z.
Snippet: The cellular properties of ATZ-CFP appear to recapitulate what is seen for ATZ in other genetically engineered cell lines, as previously described [4]. ATG5 knockdown cell lines (HTOZATG5KD) were created by transfecting HTO/Z cells with shRNA constructs (OriGene, Rockville, MD)
Aberrant SIRT6 expression contributes to melanoma growth: Role of the autophagy paradox and IGF-AKT signaling
Wang, Guo, Ma et al
Autophagy (2018) 14 (3), 518-533 DOI: 10.1080/15548627.2017.1384886
Cited by 64
AI Summary: In this study, OriGene products—specifically the SIRT6 expression plasmid (RC208307) and SIRT6 shRNA lentiviral particles (TL313763V)—were used to manipulate SIRT6 levels in melanoma cells, enabling the authors to investigate the functional role of SIRT6 in melanoma growth and its association with autophagy and IGF-AKT signaling.
Human adenovirus type 5 induces cell lysis through autophagy and autophagy-triggered caspase activity
Jiang, White, Ríos-Vicil et al
J. Virol. (2011) 85 (10), 4720-9 DOI: 10.1128/JVI.02032-10
Cited by 28
AI Summary: OriGene products contributed to this study by providing shRNA-expressing plasmids targeting ATG5 and ATG10, which were used to investigate the role of these autophagy genes in adenovirus-induced cell lysis.
Snippet: RNA interference.To knock down ATG5 and ATG10, we purchased small hairpin RNA (shRNA)-expressing plasmids from Origene (Rockville, MD). We transferred the plasmids into the cells with FuGENE 6. The cells were screened
Short-chain fatty acids induced autophagy serves as an adaptive strategy for retarding mitochondria-mediated apoptotic cell death
Tang, Chen, Jiang et al
Cell Death Differ (2011) 18 (4), 602-18 DOI: 10.1038/cdd.2010.117
Cited by 204
AI Summary: In this study, OriGene products—specifically, human ATG5 shRNA (Cat# TF312508) and human ATG7 shRNA (Cat# TF313347)—were used to genetically knock down ATG5 and ATG7, helping the researchers demonstrate that inhibition of autophagy sensitizes colon cancer cells to short-chain fatty acid-induced apoptosis.
Energy restriction as an antitumor target of thiazolidinediones
Wei, Kulp, Chen
J Biol Chem (2010) 285 (13), 9780-9791 DOI: 10.1074/jbc.M109.065466
AI Summary: In this paper, OriGene’s Sirt1 antibody (Cat# TA310106) was used to detect Sirt1 protein expression, contributing to the study’s demonstration that thiazolidinediones induce hallmark energy restriction responses in cancer cells.
Showing 1-6 of 6 papers.
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