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Positive shRNA control against tGFP (pRS Vector)

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SKU
TR30009
HuSH 29-mer shRNA against tGFP (in pRS Vector), 5 ug
  $270.00
In Stock*
Specifications
Specifications
Product Data
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
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file-alt Citations

Artesunate restores mitochondrial fusion-fission dynamics and alleviates neuronal injury in Alzheimer's disease models
Qin, Ma, Jiang et al
J Neurochem (2022)
Cited by 31
AI Summary: OriGene’s shRNA plasmid targeting OPA-1 (TR30009) was used to down-regulate mitochondrial fusion protein expression, helping the authors assess how OPA-1 reduction affects the neuroprotective effects of artesunate in Alzheimer’s disease models.
Snippet: TR30009;Origene) and a scrambled sequence targeting no known human or mouse genes (sh-NC) was used as the negative control. All plasmids were transfected into
Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion
Dukic, Gerbaud, Guibourdenche et al
Biochem. J. (2017)
AI Summary: OriGene’s turboGFP antibody was used in this study to detect GFP-tagged proteins, facilitating the analysis of connexin 43 phosphorylation and its role in gap junction assembly, communication, and cell fusion.
Snippet: Ser373) 135 (1:1000, Invitrogen), AKAP18 (1 μg/ml, Sigma-Aldrich, Courtaboeuf, France), GFP-tag (1 μg/ml, 136 Clontech, Saint Quentin, France), turboGFP (1:1000, OriGene). After incubation with appropriate 137 DyLight Fluor
Identification of Human Junctional Adhesion Molecule 1 as a Functional Receptor for the Hom-1 Calicivirus on Human Cells
Sosnovtsev, Sandoval-Jaime, Parra et al
MBio (2017) 8 (1) DOI: 10.1128/mBio.00031-17
AI Summary: OriGene’s pRG200004 plasmid, encoding a C-terminal fusion of hJAM1 and turboGFP, was used to demonstrate that expression of hJAM1 enables nonpermissive CHO cells to support Hom-1 calicivirus infection, thereby confirming hJAM1’s role as a functional viral receptor.
Snippet: Supporting the latter conclusion, expression of the C-terminal fusion of the hJAM1 protein and turboGFP (pRG200004;OriGene Technologies, Inc., Rockville, MD) (Fig. 7A) also led to productive infection of CHO cells transfected with the corresponding expression construct ... All 3 versions Cite SaveSaving
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Novel Topology of Proline-Rich Transmembrane Protein 2 (PRRT2): Hints for an Intracellular Function at the Synapse
Rossi, Sterlini, Castroflorio et al
J. Biol. Chem. (2016)
AI Summary: OriGene contributed to this study by providing the mouse PRRT2 protein fused at the C-terminus with turboGFP, which was used to investigate the protein's membrane topology and intracellular localization.
Snippet: For the GFP construct, the PRRT2 mouse coding sequence was cloned in the pEGFP-C2 plasmid (BD Bioscience). Mouse PRRT2 fused at C-terminal with turboGFP was purchased by Origene. All primers were purchased from Eurofins Genomics (Ebersberg, Germany ... Cite SaveSaving
MicroRNA-330-5p as a Putative Modulator of Neoadjuvant Chemoradiotherapy Sensitivity in Oesophageal Adenocarcinoma
Bibby, Reynolds, Maher
PLoS ONE (2015) 10 (7), e0134180 DOI: 10.1371/journal.pone.0134180
Cited by 38
AI Summary: In this study, OriGene's turboGFP mouse monoclonal antibody was used to detect turboGFP expression in Western blot analyses, aiding the investigation of molecular pathways regulated by miR-330-5p in oesophageal adenocarcinoma cells.
Snippet: Blots were probed for E2F1 (1:1000 dilution, KH95 mouse monoclonal, Santa Cruz Biotechnology, Texas, USA), turboGFP (1:10000 dilution, mouse monoclonal, Origene, Rockville, Maryland, USA) and the loading control β-actin (1:10000 dilution, AC-15 mouse monoclonal ... Cite SaveSaving
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WB
Identification and cytoprotective function of a novel nestin isoform, Nes-S, in dorsal root ganglia neurons
Su, Chen, Chang et al
J. Biol. Chem. (2013) 288 (12), 8391-404 DOI: 10.1074/jbc.M112.408179
Cited by 1
AI Summary: OriGene's nonfunctioning shRNA vector (TR30008) was used as a control in transfection experiments to assess the specific effects of Nes-S knockdown on DRG neuron survival in this study.
Snippet: For control, DRG neurons were transfected with the nonfunctioning shRNA vector (OriGene, TR30008) by the same procedure. ... The RT-PCR results showed that although a 4.5-kb band was amplified in C6, a distinct 0.5-kb major band was produced in DRG neuron RNA
Showing 1-6 of 6 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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