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Negative shRNA control in pGFP-V-RS Vector

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SKU
TR30008
HuSH 29-mer shRNA against GFP (in pGFP-V-RS Vector, Negative control), 5 ug
  $270.00
In Stock*
Specifications
Specifications
Product Data
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
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The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis
Kong, Kang, Ahn et al
IJMS (2023) 24 (23), 16704 DOI: 10.3390/ijms242316704
Cited by 9
AI Summary: OriGene’s CPNE7 shRNA (TL305242) and shRNA plasmid negative control vector (TR30008) were used to suppress CPNE7 expression in colorectal cancer cells, enabling the study to demonstrate that CPNE7 knockdown inhibits cancer cell growth, migration, and invasion.
Snippet: …The aim of this study is to determine the contribution of CPNE7 to the growth, tumorigenesis, and invasion of colorectal cancer cells, and as a result of the analysis of the patient data, it was found that the expression of CPNE7 was associated with the metastasis of CRC. … For transfections, cells were incubated with CPNE7 shRNA (ORIGENE, Rockville, MD, USA, TL305242) at 50 μmol/L concentrations in Opti MEM medium. The transfection efficiencies of cell lines were tested by transfection of the shRNA plasmid negative control vector (ORIGENE, Rockville, MD, USA, TR30008). …
Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer
Zimmer, Howell, Ma et al
Oncotarget (2021) 12 (19), 1886-1902 DOI: 10.18632/oncotarget.28059
Cited by 6
Snippet: LNCaP AD and LNCaP CR cells were transfected with plasmids encoding shRNA targeted to UGDH or a scrambled non-targeting control (UGDH shRNA-pGFP-V-RS #TG334012 and 29-mer oligo-pGFP-V-RS #TR30008, respectively, from Origene Technologies, Rockville
FigureFigureFigure
Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V
Rai, Tanaka, Suzuki et al
Nat Commun (2020) 11 (1), 4147 DOI: 10.1038/s41467-020-17666-8
Cited by 31
AI Summary: OriGene’s pGFP-V-RS expression vectors were used to generate CALM-specific and control shRNA constructs, enabling the study to investigate how CALM knockdown affects the growth of acute myeloid leukemia cells with mutant FLT3 and KIT.
Snippet: sitive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into pGFP-V-RS (OriGene Technologies, Rockville, MD). These shRNA expression vectors were transduced into Ba/F3 clones each expressing FLT3 WT, FLT3 ITD, KIT WT, and KIT D814V by a retrovirus system. shRNA-transfecte
FigureFigureFigure
USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels
Kerrisk Campbell, Sheng
J. Neurosci. (2018) 38 (23), 5289-5301 DOI: 10.1523/JNEUROSCI.3305-17.2018
AI Summary: OriGene products, including Myc-tagged rat USP8 cDNA and rat USP8 shRNA constructs, were used in this study to manipulate USP8 expression and investigate its role in regulating SHANK3 protein levels and synapse density.
Snippet: Human SHANK2 Myc-tagged pCMV, human GKAP pCMV, and Myc-tagged rat 119 USP8 cDNAs were purchased (OriGene Technologies) Rat USP8 shRNA constructs were purchased 121 (OriGene Technologies) and targeted the following sequences: USP8_A 122
RhoC regulates actin remodeling to form phagosomes during FcγR-mediated phagocytosis
Egami, Kawai, Araki
J. Cell. Sci. (2017)
Cited by 24
AI Summary: OriGene contributed RhoC shRNAs (GI557339 and GI557340) and a control shRNA (TR30008, negative control shRNA pGFP-V-RS) used for gene silencing experiments that demonstrated the essential role of RhoC in actin remodeling during FcγR-mediated phagocytosis in this study.
Snippet: RAW264 cells. RhoC shRNAs cloned into the pGFP-V-RS vector were purchased from Origene (Rockville, MD). Two different RhoC shRNAs (GI557339 and GI557340) and a control shRNA (TR30008: negative control shRNA pGFP-V-RS non-effective tGFP
IFN-λ4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
Obajemu, Rao, Dilley et al
J. Immunol. (2017)
AI Summary: OriGene provided positive and negative Western blot control lysates (HEK293 cells transfected with a SOCS1 plasmid and untransfected, respectively) to validate SOCS1 protein detection in this study.
Snippet: … Positive and negative Western blot controls (OriGene) were represented by lysates of HEK293 cells transfected with a SOCS1 plasmid or left untransfected, respectively. … Positive and negative Western blot controls (OriGene) were represented by lysates of HEK293 cells transfected with a SOCS1 plasmid or left untransfected, respectively. …
Showing 1-6 of 20 papers.
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