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Ano5 Mouse shRNA Plasmid (Locus ID 233246)

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SKU
TL509067
Ano5 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
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Specifications
Specifications
Product Data
Locus ID 233246
Synonyms 9330162L24; Gdd1; Tmem16e
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components Ano5 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 233246). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC109163, NM_001271879, NM_177694, NR_073508, NM_177694.1, NM_177694.2, NM_177694.3, NM_177694.4, NM_177694.5, NM_177694.6, NM_001271879.1
UniProt ID Q75UR0
Summary This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2012
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

Deficiency of Anoctamin 5/TMEM16E causes nuclear positioning defect and impairs Ca2+ signaling of differentiated C2C12 myotubes
Phuong, An, Park et al
Korean J. Physiol. Pharmacol. (2019) 23 (6), 539-547 DOI: 10.4196/kjpp.2019.23.6.539
AI Summary: OriGene’s pGFP-VRS ANO5 shRNA cloning plasmid was used to stably knock down ANO5 expression in C2C12 myoblasts, enabling the study of ANO5’s role in nuclear positioning and Ca2+ signaling in muscle cells.
Snippet: Stable knockdown of ANO5 in C2C12 myoblasts was established by transfecting the shANO5 construct (pGFP-VRS ANO5 shRNA cloning plasmid, OriGene Technologies, Rockville, MD, USA) by lipofectamine 2000 (Invitrogen). An shRNA target sequence for mouse Ano5 (accession number: NM_177694.6), 5ʹ-AGGAGGCGTCTTATGTTCCAGAGAAGTGA3ʹ, was selected using the OriGene shRNA design tool (construct #1
FigureFigureFigure
Showing 1-1 of 1 papers.
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