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Pak2 Mouse shRNA Plasmid (Locus ID 224105)

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SKU
TL506650
Pak2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 224105
Synonyms 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; mKIAA4182; PAK-2
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components Pak2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 224105). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC086650, NM_177326, NM_177326.1, NM_177326.2, NM_177326.3, BC029195, BC043712, BC063068
UniProt ID Q8CIN4
Summary Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (By similarity).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Simultaneous Inhibition of BCR-ABL1 Tyrosine Kinase and PAK1/2 Serine/Threonine Kinase Exerts Synergistic Effect against Chronic Myeloid Leukemia Cells
Flis, Bratek, Chojnacki et al
Cancers (Basel) (2019) 11 (10) DOI: 10.3390/cancers11101544
AI Summary: OriGene’s PAK2 mouse shRNA pRFP-C-RS vectors (TF506650) were used to specifically downregulate PAK2 in BCR-ABL1-transformed cells, enabling the study of combined kinase inhibition effects in chronic myeloid leukemia models.
Snippet: PAK2 mouse shRNA pRFP-C-RS vectors (TF506650, OriGene, MD, USA), were transfected into 32DclBCR-ABL1 cells using electroporation system GenePulser Xcell (Bio-Rad, Hercules, CA, USA). Cells expressing RFP fluorescent protein were separated and collected by the use of the FACS Aria (BD Biosciences). In addition, cells were selected and maintained in media containing 2 µg/mL puromycin
FigureFigureFigure
Showing 1-1 of 1 papers.
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