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Skp2 Mouse shRNA Plasmid (Locus ID 27401)

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SKU
TL502703
Skp2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 27401
Synonyms 4930500A04Rik; FBXL1; FWD1; p45
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components Skp2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 27401). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC092236, NM_001285980, NM_013787, NM_145468, NM_013787.1, NM_013787.2, NM_013787.3, NM_145468.1, NM_001285980.1, BC003468, BC098371
UniProt ID Q9Z0Z3
Summary Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Down-regulation of Skp2 expression inhibits invasion and lung metastasis in osteosarcoma
Zhang, Zvi, Batko et al
Sci Rep (2018) 8 (1), 14294 DOI: 10.1038/s41598-018-32428-9
AI Summary: OriGene products, including the HuSH shRNA control plasmid pGFP-V-RS (TR30013) and two 29-mer HuSH shRNA plasmids targeting Skp2 (SR073262 / TG301684A-D), were used in this study to knock down Skp2 expression in osteosarcoma cells, enabling the investigation of Skp2’s role in tumor invasion and metastasis.
Snippet: … A HuSH shRNA control plasmid pGFP-V-RS with scrambled shRNA sequence (TR30013) and two 29-mer HuSH shRNA plasmids targeting Skp2 (SR073262 / TG301684A-D) were purchased from Origene (Rockland, MD). Cells were transiently transfected with 8 µg of the indicated plasmids using Lipofectamine 2000, incubated overnight, and selected with 2 µg/mL puromycin for 48 hours.;A HuSH shRNA control plasmid pGFP-V-RS with scrambled shRNA sequence (TR30013) and two 29-mer HuSH shRNA plasmids targeting Skp2 (SR073262 / TG301684A-D) were purchased from Origene (Rockland, MD). …
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