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Ube2e1 Mouse shRNA Plasmid (Locus ID 22194)

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SKU
TL502364
Ube2e1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*

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Other products for "Ube2e1 or NM_009455"

Specifications
Specifications
Product Data
Locus ID 22194
Synonyms Ubce5; ubcM2; UbcM3
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components Ube2e1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22194). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC003781, NM_009455, NM_009455.1, NM_009455.2, NM_009455.3
UniProt ID P52482
Summary Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1
Szymanska, Boldt, Logan et al
Elife (2022) 11 DOI: 10.7554/eLife.57593
AI Summary: In this study, OriGene's Ube2e1 shRNA (catalog number TR502364) was used to knock down Ube2e1 expression in cells, enabling the authors to investigate the role of UBE2E1 in ciliary function and Wnt signalling.
Snippet: this paper c.341T > A, p.C131S UBE2E1 active site dominant negative (DN) mutation;see Cloning, plasmid constructs and transfection Transfected construct (Mus musculus) Ube2e1 shRNA Origene TR502364 Cells selected using 0.5 μg/ml puromycin for five passages Transfected construct (Mus musculus) Ube2e1 siRNA Dharmacon ON-TARGET PLUS siRNA L-062416-01-0005 Tr;musculus) Ube2e1 shRNA Origene TR502364 Cells selected using 0.5 μg/ml puromycin for five passages
FigureFigureFigure
Showing 1-1 of 1 papers.
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