NELF (NSMF) Human shRNA Plasmid Kit (Locus ID 26012)

SKU
TL317072
NSMF - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*
Specifications
Specifications
Product Data
Locus ID 26012
Synonyms HH9; NELF
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components NSMF - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 26012). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001130969, NM_001130970, NM_001130971, NM_001178064, NM_015537, NM_182780, NM_015537.1, NM_015537.2, NM_015537.3, NM_001130971.1, NM_001130970.1, NM_001130969.1, BC072412, BC072412.1, BC110498, BC004318, BC016862, BM023184, NM_001130969.3
UniProt ID Q6X4W1
Summary The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2010
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

RNAP II processivity is a limiting step for HIV-1 transcription independent of orientation to and activity of endogenous neighboring promoters
Michaels, Wolschendorf, Lester
Virology (2015)
AI Summary: OriGene’s NELF shRNA and control scrambled shRNA were used in this study to manipulate gene expression in HEK293T cells, enabling the authors to investigate the role of RNAP II processivity in HIV-1 transcription.
Snippet: Transfections, virus generation and infections. NELF shRNA and control scrambled shRNA (OriGene) were packaged by cotransfecting Tat, RSV-Rev, Gag/Pol and VSV-G into HEK293T cells using calcium phosphate as previously described (Natarajan et al., 2013) ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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