PDHA1 Human shRNA Plasmid Kit (Locus ID 5160)
SKU
TL310532
PDHA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
$1,013.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 5160 |
|---|---|
| Synonyms | PDHA; PDHAD; PDHCE1A; PHE1A |
| Vector | pGFP-C-shLenti |
| E. coli Selection | Chloramphenicol (34 ug/ml) |
| Mammalian Cell Selection | Puromycin |
| Format | Lentiviral plasmids |
| Components | PDHA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5160). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
| RefSeq | NM_000284, NM_001173454, NM_001173455, NM_001173456, NM_000284.1, NM_000284.2, NM_000284.3, NM_001173456.1, NM_001173455.1, NM_001173454.1, BC002406, BC002406.1, NM_001173456.2, NM_001173454.2, NM_000284.4, NM_001173455.2 |
| UniProt ID | P08559 |
| Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Mar 2010 |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Pyruvate Dehydrogenase Contributes to Drug Resistance of Lung Cancer Cells Through Epithelial Mesenchymal Transition
Cevatemre, Ulukaya, Dere et al
Front Cell Dev Biol (2021) 9, 738916 DOI: 10.3389/fcell.2021.738916
Cevatemre, Ulukaya, Dere et al
Front Cell Dev Biol (2021) 9, 738916 DOI: 10.3389/fcell.2021.738916
Cited by 19
AI Summary: OriGene’s PDHA1 Human shRNA Plasmid Kit (TL310532) was used to genetically knock down PDHA1 in lung cancer cells, enabling the study to investigate the role of PDH in promoting epithelial mesenchymal transition and drug resistance.
Snippet: osphere with 5% CO2. 2.2 Knockdown of PDHA1 Using shRNA PDHA1 human shRNA lentiviral vector plasmid and the corresponding control backbone (PDHA1 Human shRNA Plasmid Kit, TL310532) was obtained from OriGene Technologies. Lentivirus was produced by co-transfecting the lentiviral vector, the packaging plasmid (PAX2), and the envelope plasmid (pMD2.G) into HEK-293T cells using Lipofectamine 3000;PDHA1 human shRNA lentiviral vector plasmid and the corresponding control backbone (PDHA1 Human shRNA Plasmid Kit, TL310532) was obtained from OriGene Technologies
Showing 1-1 of 1 papers.
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