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Pparg Rat shRNA Plasmid (Locus ID 25664)

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SKU
TG709717
Pparg - Rat, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 25664
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Pparg - Rat, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 25664). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001145366, NM_001145367, NM_013124, NM_013124.1, NM_013124.2, NM_013124.3, NM_001145366.1, NM_001145367.1
UniProt ID O88275
Summary ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism RGD, Feb 2006
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

SetD7 (Set7/9) is a novel target of PPARγ that promotes the adaptive pancreatic β-cell glycemic response
Jetton, Flores-Bringas, Leahy et al
J Biol Chem (2021) 297 (5), 101250 DOI: 10.1016/j.jbc.2021.101250
Cited by 15
AI Summary: OriGene's PPARγ shRNA and scrambled control shRNA were used to generate stable INS-1 β-cell lines with reduced PPARγ expression, enabling the authors to demonstrate that PPARγ directly regulates SetD7 and Pdx1 expression in pancreatic β-cells.
Snippet: Rγ. We next tested direct regulation of SetD7 by PPARγ in INS-1 cells with a constitutively KD of PPARγ. We established and characterized GFP-tagged stable constitutive expression of PPARγ shRNA (Origene) and scrambled control (scr) in INS-1 β-cells (Fig. 2Ai). We obtained an shRNA clone that reduced PPARγ protein expression to 0.37-fold and correspondingly reduced Pdx1 (0.6-fold) and SetD7
FigureFigureFigure
Showing 1-1 of 1 papers.
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