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Ezh2 Mouse shRNA Plasmid (Locus ID 14056)

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SKU
TG509722
Ezh2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 14056
Synonyms Enx-1; Enx1h; KMT6; mKIAA4065
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Ezh2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 14056). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq BC003772, BC016391, BC079538, NM_001146689, NM_007971, NM_007971.1, NM_007971.2, NM_001146689.1
UniProt ID Q61188
Summary Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

Maternal regulation of SATB2 in osteo-progeniters impairs skeletal development in offspring
Chen, Zhao, Lazarenko et al
FASEB J (2020) 34 (2), 2511-2523 DOI: 10.1096/fj.201901901R
AI Summary: OriGene products contributed to this study by providing the Ezh2 shRNA (Cat# TL708334) and Ezh2 overexpression plasmid (Cat# RC217434), which were used to manipulate Ezh2 expression levels and assess their effects on SATB2 regulation and osteoblast differentiation.
Maternal regulation of SATB2 in osteo‐progeniters impairs skeletal development in offspring
Chen, Zhao, Lazarenko et al
FASEB j. (2019)
AI Summary: In this study, OriGene’s Ezh2 shRNA (Ezh2-sh, #TG509722) was used to knock down Ezh2 expression in cells, helping demonstrate the role of Ezh2 in regulating SATB2 and osteoblast differentiation in the context of maternal obesity.
Snippet: Cells were treated with a mix- ture of NEFA at 400 µM (palmitate and oleic 1:2 mixture) for 48 hours. Ezh2 overexpression plasmid (Ezh2, #28060, Addgene) and shRNA Ezh2 (Ezh2-sh, #TG509722, Origene) were used. According
HOTAIR-mediated reciprocal regulation of EZH2 and DNMT1 contribute to polyphyllin I-inhibited growth of castration-resistant prostate cancer cells in vitro and in vivo
Xiang, Zou, Tang et al
Biochim. Biophys. Acta (2017) 1862 (3), 589-599 DOI: 10.1016/j.bbagen.2017.12.001
Snippet: and PC3 cells seeded in 96-well plates (5 × 10 3 cells/well) were incubated at 37 °C with 5% CO 2 with increasing concentrations of PPI for up to 72 h. In separate experiments, NSCLC cells were transfected with control, pCMV6-EZH2 obtained from OriGene Technologies
Showing 1-3 of 3 papers.
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