Upf1 Mouse shRNA Plasmid (Locus ID 19704)
SKU
TG501884
Upf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 19704 |
|---|---|
| Synonyms | B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp |
| Vector | pGFP-V-RS |
| E. coli Selection | Kanamycin |
| Format | Retroviral plasmids |
| Components | Upf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 19704). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
| RefSeq | BC052149, BC056442, NM_001122829, NM_030680, NM_030680.1, NM_030680.2, NM_030680.3, NM_001122829.1, NM_001122829.2, BC030916, BC038921 |
| UniProt ID | Q9EPU0 |
| Summary | RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Shipping | Ambient |
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