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ELMO1 Human shRNA Plasmid Kit (Locus ID 9844)

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SKU
TG320622
ELMO1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 9844
Synonyms CED-12; CED12; ELMO-1
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components ELMO1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 9844). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001039459, NM_001206480, NM_001206482, NM_014800, NM_130442, NR_038120, NR_038121, NM_014800.1, NM_014800.10, NM_014800.2, NM_014800.3, NM_014800.4, NM_014800.5, NM_014800.6, NM_014800.7, NM_014800.9, NM_001039459.1, NM_001039459.2, NM_130442.1, NM_130442.2, NM_130442.3, NM_001206480.1, NM_001206480.2, NM_001206482.1, BC114341, BC003051, BC064635, BC077074
UniProt ID Q92556
Summary This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis
Arandjelovic, Perry, Lucas et al
Nat. Immunol. (2019) 20 (2), 141-151 DOI: 10.1038/s41590-018-0293-x
AI Summary: OriGene’s human Elmo1-targeting and control shRNA pGFP-V-RS expression vectors were used to generate Elmo1 knockdown HL-60 cells, enabling the study of ELMO1’s role in neutrophil migration and inflammatory arthritis.
Snippet: … For generating _Elmo1_ knockdown cells, HL-60 cells were transfected with 10 μg of human _Elmo1_ targeting or control shRNA pGFP-V-RS expression vectors (OriGene Technologies, Inc.) using electroporation using a 250 V, 25 ms pulse. … For generating Elmo1 knockdown cells, HL-60 cells were transfected with 10 μg of human Elmo1 targeting or control shRNA pGFP-V-RS expression vectors (OriGene Technologies, Inc.) using electroporation using a 250 V, 25 ms pulse. … For generating Elmo1-knockdown cells, HL-60 cells were transfected with 10 μg of human Elmo1-targeting or control short hairpin RNA (shRNA) pGFP-V-RS expression vectors (OriGene Technologies) bby electroporation using a 250 V, 25 ms pulse
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