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PTEN Human shRNA Plasmid Kit (Locus ID 5728)

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SKU
TG320498
PTEN - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 5728
Synonyms 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; TEP1
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components PTEN - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 5728). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_000314, NM_001304717, NM_001304718, NM_000314.1, NM_000314.2, NM_000314.3, NM_000314.4, NM_000314.5, NM_000314.6, BC005821, NM_000314.7
UniProt ID P60484
Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. provided by RefSeq, Feb 2015
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

F-box and leucine-rich repeat protein 5 promotes colon cancer progression by modulating PTEN/PI3K/AKT signaling pathway
Yao, Su, Xia et al
Biomed. Pharmacother. (2018) 107, 1712-1719 DOI: 10.1016/j.biopha.2018.08.119
AI Summary: OriGene products, specifically the human FBXL5 gene plasmid (No. TF304567) and the human PTEN gene plasmid (No. TG320498) along with their controls, were used in this study to investigate the roles of FBXL5 and PTEN in colon cancer progression via the PTEN/PI3K/AKT signaling pathway.
Snippet: No. TF304567) and human PTEN gene (No. TG320498), and their controls were purchased from OriGene (MA, USA). The overexpressed plasmid of FBXL5 (OE-FBXL5) were obtained from GenePharma (Shanghai, China)
Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a
Xin, Wu, Meng et al
Mol. Cancer (2018) 17 (1), 94 DOI: 10.1186/s12943-018-0843-8
AI Summary: OriGene products, including the plasmids pGFP-V-RS, pCMV6-A-GFP, pCMV6-XL5-PTEN, pGFP-V-RS-PTEN, pGFP-V-RS-Sirt1, and pMiR-Target, were used in this study to manipulate gene expression and investigate the molecular mechanisms by which lncRNA HULC accelerates liver cancer progression.
Snippet: with 10% heat-inactivated fetal bovine serum (Gibco) in a humidified atmosphere of 5% CO 2 incubator at 37 °C. Plasmid pGFP-V-RS, pCMV6-A-GFP, pCMV6-XL5-PTEN, pGFP-V-RS-PTEN, pGFP-V-RS-Sirt1, pMiR-Target were purchased from Origene (Rockville Cached
FigureFigureFigure
Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting β-catenin by activating PKM2 and inactivating PTEN
Zheng, Lin, Xu et al
Cell Death Dis (2018) 9 (3), 253 DOI: 10.1038/s41419-018-0305-7
AI Summary: OriGene contributed to this study by providing the plasmids pGFP-V-RS, pCMV6-A-GFP, pCMV6-XL5-β-catenin, pCMV6-XL5-PTEN, pGFP-V-RS-PTEN, pGFP-V-RS-β-catenin, and pMiR-Target, which were used for gene expression and functional analysis in liver cancer cells.
Snippet: bovine serum (FBS) (Gibco) in a humidified atmosphere of 5% CO 2 incubator at 37 °C. Plasmids pGFP-V-RS, pCMV6-A-GFP, pCMV6-XL5-β-catenin, pCMV6-XL5-PTEN, pGFP-V-RS-PTEN, pGFP-V-RS-β-catenin, and pMiR-Target were purchased from Origene;
Avian Reovirus Protein p17 Functions as a Nucleoporin Tpr Suppressor Leading to Activation of p53, p21 and PTEN and Inactivation of PI3K/AKT/mTOR and ERK Signaling Pathways
Huang, Chiu, Liao et al
PLoS ONE (2015) 10 (8), e0133699 DOI: 10.1371/journal.pone.0133699
Cited by 47
AI Summary: OriGene products contributed to this study by providing all the shRNAs and scrambled negative shRNA used for gene knockdown experiments to investigate the roles of Tpr, p53, PTEN, and other related proteins.
Snippet: The AKT DN mutant was kindly provided by Professor Shih, National Pingtung University of Science and Technology, Taiwan. In the present study, all shRNAs and scrambled negative shRNA (Table 2) purchased from OriGene Co. ... Cite SaveSaving
FigureFigureFigure
RIP1 Kinase Is an Oncogenic Driver in Melanoma
Liu, Lai, Yan et al
Cancer Res. (2015)
AI Summary: OriGene products contributed to this study by providing the shRNA kit (TG300130) for RIP1 gene silencing and the pCMV-Myc-RIP1 construct for RIP1 overexpression, enabling the authors to investigate RIP1's role in melanoma cell proliferation and tumor growth.
Snippet: shRNA kit (TG300130), in which shRNA constructs are in retroviral GFP vector (pGFP-VRS), were purchased from Origene, and were used according to manufacturer's protocols. ... Vector Kit) was purchased from Invitrogen. The pCMV-Myc-RIP1 construct was from Origene ... Cite SaveSaving
PI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma
Ye, Jin, Wilmott et al
Nat Commun (2013) 4, 1508 DOI: 10.1038/ncomms2489
Cited by 75
AI Summary: OriGene products contributed to this study by providing the PIB5PA cDNA (OriGene, RC204307) for overexpression experiments and the PIB5PA shRNA (OriGene, TG313019) for gene knockdown, enabling the authors to assess the tumour suppressive role of PIB5PA in melanoma cells.
Snippet: Quantification of each band was determined using NIH Image J. (e) Comparison of pSer473-Akt levels between fresh melanoma isolates negative for both PIB5PA and PTEN, and those expressing at least one of the proteins.
FigureFigureFigure
Showing 1-6 of 8 papers.
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