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HDAC2 Human shRNA Plasmid Kit (Locus ID 3066)

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SKU
TG312495
HDAC2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 3066
Synonyms HD2; KDAC2; RPD3; YAF1
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components HDAC2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 3066). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001527, NR_033441, NR_073443, NM_001527.1, NM_001527.2, NM_001527.3, BC013141, BC031055, BC146376, BC148797, BM550464
UniProt ID Q92769
Summary This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2010
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Precision Targeting with EZH2 and HDAC Inhibitors in Epigenetically Dysregulated Lymphomas
Lue, Prabhu, Liu et al
Clin. Cancer Res. (2019)
AI Summary: OriGene’s Human HDAC2 shRNA plasmids (#TG312495) were used to generate stable HDAC2 knockdown cell lines, enabling the study of HDAC2’s role in the synergistic effects of EZH2 and HDAC inhibition in lymphoma models.
Snippet: Human HDAC2 shRNA plasmids were purchased from Origene (#TG312495). HEK293 cells were plated in OPTI-MEM containing shRNA or scramble using Lipofectamine 3000 (Cat#L3000075). Cells were selected with puromycin, periodically analyzed by flow cytometry and fluorescent microscopy to monitor GFP levels until a stable cell line had been generated
LPS impairs oxygen utilization in epithelia by triggering degradation of the mitochondrial enzyme Alcat1
Zou, Synan, Li et al
J. Cell. Sci. (2015)
Cited by 20
AI Summary: In this study, OriGene products contributed by providing Fbxo28 and HDAC2 shRNA constructs as well as Alcat1 cDNA, which were essential for gene silencing and expression experiments investigating the regulation and degradation of the mitochondrial enzyme Alcat1.
Snippet: Fbxo28 and HDAC2 shRNA constructs were purchased from Origene. ... Page 20. Construction of plasmids and cell transfection - The series truncation mutations of Alcat1 were constructed by PCR using Alcat1 cDNA (Origene) as template and following primers: Alcat1 ... Cite SaveSaving
HDAC2-dependent Deacetylation of MORF4L1 Enhances Its Homodimerization
Chen, Li, Dunn et al
J. Biol. Chem. (2014)
AI Summary: In this study, OriGene products contributed the HDAC2 shRNA retroviral plasmid and human MORF4L1 cDNA, which were used to investigate the roles of HDAC2 and MORF4L1 in regulating MORF4L1 homodimerization.
Snippet: HDAC2 small hairpin RNA (shRNA) retroviral plasmid was from Origene (Rockville, MD). ... Human MORF4L1 cDNA was purchased from Origene, and the coding region of the gene was cloned into pcDNA 3.1 by PCR using the forward primer: 5'- ... Cite SaveSaving
Histone Deacetylase 2 (HDAC2) Protein-dependent Deacetylation of Mortality Factor 4-like 1 (MORF4L1) Protein Enhances Its Homodimerization
Chen, Li, Dunn et al
Journal of Biological Chemistry (2014)
AI Summary: OriGene contributed to the study by providing the HDAC2 shRNA retroviral plasmid, which was used for gene knockdown experiments to investigate the role of HDAC2 in MORF4L1 protein deacetylation and homodimerization.
Snippet: Acetyl-lysine antibody, HDAC2 antibody, and PF1 antibody were from Sigma. HDAC2 shRNA retroviral plasmid was from Origene (Rockville, MD). Plasmid Expression and shRNA Knockdown. Cells were nucleofected with plasmids as described previously (16). ... Cite SaveSaving
Nuclear Localization of the Transcriptional Regulator MIER1α Requires Interaction with HDAC1/2 in Breast Cancer Cells
Li, Paterno, Gillespie
PLoS ONE (2013) 8 (12), e84046 DOI: 10.1371/journal.pone.0084046
Cited by 15
AI Summary: OriGene products contributed to this study by providing plasmids containing ERα shRNA, HDAC1 shRNA, HDAC2 shRNA, and a control scrambled shRNA, which were used to investigate the roles of these proteins in the nuclear localization of MIER1α.
Snippet: Plasmids containing ERα shRNA, HDAC1 shRNA, HDAC2 shRNA or a control scrambled shRNA were purchased from Origene Technologies, Inc. Antibodies. The 9E10 anti-myc tag mouse monoclonal antibody was prepared as described in Blackmore et al. ... Cite SaveSaving
Showing 1-5 of 5 papers.
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