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SMAD4 Human shRNA Plasmid Kit (Locus ID 4089)

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SKU
TG309253
SMAD4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
In Stock*

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Other products for "SMAD4 or NM_005359"

Specifications
Specifications
Product Data
Locus ID 4089
Synonyms DPC4; JIP; MADH4; MYHRS
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components SMAD4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 4089). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_005359, NM_005359.1, NM_005359.2, NM_005359.3, NM_005359.4, NM_005359.5, BC002379, BC002379.2, BM701399, NM_005359.6
UniProt ID Q13485
Summary This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. provided by RefSeq, Aug 2017
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

Loss of Trefoil Factor 2 From Pancreatic Duct Glands Promotes Formation of Intraductal Papillary Mucinous Neoplasms in Mice
Yamaguchi, Mino-Kenudson, Liss et al
Gastroenterology (2016)
Cited by 50
AI Summary: In this study, OriGene’s SMAD4-shRNA (pGFP-V-RS) was used to knock down SMAD4 expression in pancreatic cancer cell lines to investigate the role of SMAD4 in TFF2-mediated tumor suppression.
Snippet: Control vector (pCMV-SPORT6), TFF2-overexpression plasmid (Thermo Fisher Scientific), control shRNA vector (pGFP-V-RS), and SMAD4-shRNA (OriGene) were transfected into PDAC cell lines using Effectene (Qiagen) according to the manufacturer's protocol. ... Cite SaveSaving
The transcription factor GLI1 interacts with SMAD proteins to modulate TGFβ-induced gene expression in a PCAF-dependent manner
Nye, Almada, Fernandez-Barrena et al
J. Biol. Chem. (2014)
AI Summary: OriGene products contributed shRNAs targeting SMAD4 (TG309253-5, TG309253-7), SMAD2 (TG309255C, TG309255D), and a Scramble control (TR30013) for RNAi experiments that were essential to investigate the roles of these proteins in TGFβ- and GLI1-mediated gene regulation in the study.
Snippet: The shRNAs targeting SMAD4 (TG309253-5 and TG309253- 7), SMAD2 (TG309255C and TG309255D), and Scramble control (TR30013) were from Origene Page 3. Mechanism of GLI1-dependent gene expression 3 (Rockville, MD). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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