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Pclo Mouse shRNA Plasmid (Locus ID 26875)

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SKU
TF502633
Pclo - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 26875
Synonyms Acz; Pico
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Pclo - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 26875). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001110796, NM_011995
Summary Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms
Swarnkar, Avchalumov, Raveendra et al
Sci Rep (2018) 8 (1), 17419 DOI: 10.1038/s41598-018-35634-7
AI Summary: OriGene’s 29mer shRNA constructs (catalog numbers TG501174, TG501180, TG501181, TG517925, TR30013, and TF502633) were used to knock down specific Kif genes in mouse hippocampal neurons, enabling the study to uncover the roles of Kif11 and Kif21B as inhibitory regulators of excitatory synaptic transmission.
Snippet: …Mouse, 4 unique 29mer shRNA constructs in eGFP or RFP vector obtained from OriGene (TG501174, TG501180, TG501181, TG517925, TR30013, and TF502633). siRNA/shRNA were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofecta;Mouse SMARTpool siRNA reagent against all the Kifs was obtained from Dharmacon GE Healthcare. Mouse, 4 unique 29mer shRNA constructs in eGFP or RFP vector obtained from OriGene (TG501174, TG501180, TG501181, TG517925, TR30013, and TF502633). siRNA/shRNA were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines
FigureFigureFigure
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