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Yy1 Mouse shRNA Plasmid (Locus ID 22632)

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SKU
TF502443
Yy1 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 22632
Synonyms AW488674; NF-E1
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Yy1 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 22632). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq BC055899, NM_009537, NM_009537.1, NM_009537.2, NM_009537.3, NM_009537.4
UniProt ID Q00899
Summary Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. Binds to the upstream conserved region (UCR) (5'-CGCCATTTT-3') of Moloney murine leukemia virus (MuLV). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Involved in spermatogenesis and may play a role in meiotic DNA double-strand break repair. Plays a role in regulating enhancer activation (By similarity).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

Yin Yang 1 is critical for mid-hindbrain neuroepithelium development and involved in cerebellar agenesis
Dong, Kwan
Mol Brain (2020) 13 (1), 104 DOI: 10.1186/s13041-020-00643-z
Cited by 24
AI Summary: OriGene’s tagged mouse Yy1 overexpression plasmid (pCMV6-Yy1) was used in dual luciferase reporter assays to study the transcriptional activity of Yy1 in this paper.
Snippet: … Tagged mouse _Yy1_ overexpression plasmid was purchased from Origene, pCMV6-entry empty vector was used as the negative control for co-transfection. … CHROMATIN-IMMUNOPRECIPITATI;- The highly conserved and ubiquitously expressed transcription factor Yin Yang 1 (Yy1), was named after its dual functions of both activating and repressing gene transcription. Yy1 plays complex roles in various fundamental biological processes such as the cell cycle Cached;. … Tagged mouse _Yy1_ overexpression plasmid was purchased from Origene, pCMV6-entry empty vector was used as the negative control for co-transfection. …Tagged mouse Yy1 overexpression plasmid was purchased from Origene, pCMV6-entry empty vector was used as the negative control for co-transfection. …
FigureFigureFigure
The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia
Fernando, Contreras, Zampini et al
Mol. Cancer (2017) 16 (1), 126 DOI: 10.1186/s12943-017-0692-x
AI Summary: OriGene’s pCMV-YY1 expression plasmid (OriGene-SC118004) was used to overexpress the YY1 transcription factor in HEK 293T cells, enabling the authors to investigate how CASC15 and YY1 together regulate SOX4 promoter activity in acute leukemia.
Snippet: HEK 293 T cells were transfected with the pGL4.75 and pGL4.11 containing reporter vectors at a 1:10 ratio (10 ng:100 ng), along with a combination of pCMV (empty or YY1: Origene-SC118004) and MSCV (empty or CASC15) vectors at a 1:5 ratio (10 ng:50 ng), or a ... … HEK 293 T cells were transfected with the pGL4.75 and pGL4.11 containing reporter vectors at a 1:10 ratio (10 ng:100 ng), along with a combination of pCMV (empty or YY1: Origene-SC118004) and MSCV (empty or CASC15) vec- tors at a 1:5 ratio (10 ng:50 ng), or ... … HEK 293 T cells were transfected with the pGL4.75 and pGL4.11 containing reporter vectors at a 1:10 ratio (10 ng:100 ng), along with a combination of pCMV (empty or YY1: Origene-SC118004) and MSCV (empty or CASC15) vectors at a 1:5 ratio (10 ng:50 ng), or a ... …
FigureFigureFigure
Showing 1-2 of 2 papers.
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