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JAK2 Human shRNA Plasmid Kit (Locus ID 3717)

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SKU
TF320395
JAK2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 3717
Synonyms JTK10
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components JAK2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 3717). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001322198, NM_001322199, NM_004972, NM_001322194, NM_001322195, NM_001322196, NM_001322204, NM_004972.1, NM_004972.2, NM_004972.3, BC039695, BC043187, NM_004972.4
UniProt ID O60674
Summary This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2020
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

An activity-dependent determinant of synapse elimination in the mammalian brain
Yasuda, Nagappan-Chettiar, Johnson-Venkatesh et al
Neuron (2021) 109 (8), 1333-1349.e6 DOI: 10.1016/j.neuron.2021.03.006
Cited by 83
AI Summary: OriGene’s HuSH shRNA constructs in the pRFP-C-RS shRNA vector were used to express JAK2-targeting shRNA, enabling the study of JAK2’s role in synapse elimination in this paper.
Snippet: Short-hairpin RNA (shRNA) to JAK2 was expressed by HuSH shRNA constructs in pRFP-C-RS shRNA vector (Origene Technologies, Rockville, MD, USA)
Showing 1-1 of 1 papers.
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