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Adenosine Receptor A2a (ADORA2A) Human shRNA Plasmid Kit (Locus ID 135)

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SKU
TF314920
ADORA2A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 135
Synonyms A2aR; ADORA2; RDC8
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components ADORA2A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 135). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_000675, NM_001278497, NM_001278498, NM_001278499, NM_001278500, NR_103543, NR_103544, NM_000675.1, NM_000675.2, NM_000675.3, NM_000675.4, NM_001278497.1, NM_001278498.1, NM_001278499.1, NM_001278500.1, BC013780, BC013780.1, NM_000675.6
UniProt ID P29274
Summary This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. provided by RefSeq, Jun 2013
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Targeting the adenosine 2A receptor enhances chimeric antigen receptor T cell efficacy
Beavis, Henderson, Giuffrida et al
J. Clin. Invest. (2017) 127 (3), 929-941 DOI: 10.1172/JCI89455
AI Summary: OriGene’s pRFP-C-RS A2AR-targeted shRNA plasmid was used in this study to genetically knock down A2AR expression, enabling the authors to investigate the impact of A2AR suppression on CAR T cell efficacy.
Snippet: 2 × 106 HEK293gp cells were plated onto poly-d-lysine–treated 10-cm2 dishes overnight and transfected the following day with 10 μg pMD2.G, 4 μg pcSUPER-shDGCR8 (Luke Dow, Weill Cornell Medical College, New York, New York, USA) (66), and 20 μg of A2AR-targeted shRNA plasmid (pRFP-C-RS;Origene) using Lipofectamine 2000 (Invitrogen) per the manufacturer’s instructions
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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