OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ULK1 Human shRNA Plasmid Kit (Locus ID 8408)

Be the first to review this product
SKU
TF308491
ULK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
In Stock*

Frequently Bought Together (2)

TA383092 ULK1 Rabbit polyclonal Antibody
100 ul
$410.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "ULK1 or NM_003565"

Specifications
Specifications
Product Data
Locus ID 8408
Synonyms ATG1; ATG1A; hATG1; UNC51; Unc51.1
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components ULK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 8408). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_003565, NM_003565.1, NM_003565.2, BC111603, NM_003565.4
UniProt ID O75385
Summary Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Dissecting the pathways that destabilize mutant p53: the proteasome or autophagy?
Choudhury, Kolukula, Preet et al
Cell Cycle (2013) 12 (7), 1022-9 DOI: 10.4161/cc.24128
Cited by 88
AI Summary: In this study, OriGene’s Ulk1 shRNA plasmid (#TF308491) was used to downregulate Ulk1, helping to demonstrate that inhibition of autophagy leads to stabilization of mutant p53, thereby elucidating autophagy’s role in mutant p53 degradation.
Snippet: Figure 3. Autophagy controls mutant p53 expression levels. (A) p53 expression and half-life were studied in stable MDA-231 cells harboring ... The shRNA plasmids for ATG5 were purchased from Genecopia (#HSH022804-mU6), while Ulk1 was from Origene (#TF308491).
Dietary downregulation of mutant p53 levels via glucose restriction: mechanisms and implications for tumor therapy
Rodriguez, Choudhury, Kolukula et al
Cell Cycle (2012) 11 (23), 4436-46 DOI: 10.4161/cc.22778
Cited by 115
AI Summary: In this study, OriGene’s Ulk1 shRNA plasmid (#TF308491) was used to investigate the role of Ulk1 in autophagy during glucose restriction-induced degradation of mutant p53.
Snippet: nvitrogen). Sequence of siRNAs or shRNAs. The shRNA plasmids for ATG5, ATG7 were purchased from Genecopia (#HSH022804-mU6 and HSH000641-mU6, respectively, while Ulk1 was from Origene (#TF308491). Four
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products