OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Pdk1 Rat shRNA Lentiviral Particle (Locus ID 116551)

Be the first to review this product
SKU
TL712182V
Pdk1 - Rat shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.
  $1,731.00
5 Weeks*

Frequently Bought Together (2)

TR30038 One-Wash Lentivirus Titer Kit, HIV-1 p24 ELISA
96 reactions
$700.00
TR30021V Control Lenti particles, Scrambled shRNA, Expressing GFP and Puro, 1x10^7 TU/ml, 0.5 ml
1 x 10^7 TU/ml, 500 ul
$380.00

Other products for "Pdk1 or NM_053826"

Specifications
Specifications
Product Data
Locus ID 116551
Synonyms MGC108625
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral particles
Components Pdk1 - Rat shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.
RefSeq NM_053826, NM_053826.2, BC089783
Summary catalyzes the phosphorylation and inactivation of the pyruvate dehydrogenase complex RGD, Feb 2006
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
Storage Store at -80°C
Shipping Dry Ice
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes
Atser, Wenyonu, Rowe et al
J Biol Chem (2025) 301 (4), 108398 DOI: 10.1016/j.jbc.2025.108398
Cited by 7
AI Summary: OriGene’s Pdk1 shRNA Lentiviral particles (Cat. No. TL712182V) were used to knock down Pdk1 expression in cardiomyocyte cell cultures, enabling the study of PDK1's role in regulating triacylglycerol hydrolysis.
Snippet: 5% CO2 cell culture incubator. Pdk1 shRNA Lentiviral particles (Cat. No. TL712182V, Origene) were mixed with 8 μg/ml of polybrene (Cat. No. TR-1003, Sigma-Aldrich) and added to cultured cells at a multiplicity of infection (MOI) of 500 or 120 (for the scrambled shRNA control)
Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes
Atser, Wenyonu, Rowe et al
bioRxiv (2024)
Snippet: omycin and 0.25 μg/mL of Amphotericin B (Cat. No. 15240062, ThermoFisher Scientific, Burnaby, BC, CA) in a 37 °C, 5% CO2 cell culture incubator. Pdk1 shRNA Lentiviral particles (Cat. No. TL712182V, Origene, Rockville, Maryland, US) were mixed with 8 μg/mL of polybrene (Cat. No. TR-1003, Sigma-Aldrich, Oakville, ON, CA) and added to cultured cells at a multiplicity of infection (MOI) of 500 or 1
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products