Med19 Rat shRNA Lentiviral Particle (Locus ID 311165)

SKU
TL706290V
Med19 - Rat shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.
  $1,731.00
5 Weeks*
Specifications
Specifications
Product Data
Locus ID 311165
Synonyms RGD1311926
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral particles
Components Med19 - Rat shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.
RefSeq NM_001107741, NM_001107741.1
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
Storage Store at -80°C
Shipping Dry Ice
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Documents
Resources

file-alt Citations

MED19 alters AR occupancy and gene expression in prostate cancer cells, driving MAOA expression and growth under low androgen
Weber, Ruoff, Garabedian
PLoS Genet (2021) 17 (1), e1008540 DOI: 10.1371/journal.pgen.1008540
Cited by 29
AI Summary: In this study, OriGene’s MED19 Myc-DDK-tagged ORF clone (RC207063) and MED19 shRNA (TL313627) were used to overexpress and deplete MED19 in prostate cancer cells, respectively, enabling the authors to investigate MED19’s role in regulating AR activity, gene expression, and androgen-independent growth.
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Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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