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Mir101a Mouse MicroRNA Expression Plasmid (MI0000148)

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SKU
SC400719
Expression plasmid for mouse microRNA Mir101a
  $424.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Species Mouse
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: AGGCUGCCCUGGCUCAGUUAUCACAGUGCUGAUGCUGUCCAUUCUAAAGGUACAGUACUGUGAUAACUGAAGGAUGGCAGCCA:
Mature Sequence: UCAGUUAUCACAGUGCUGAUGC:
Full Insert
>MI0000148 TGTAAGATGTGCATATAACCAGAAAGAAAGTTCACTTGTGCTTACTCCATTTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGTGTTCACCTTTCCTTTACTGTA
GTTCAATTGGTCTTTACTGCTTCACCCTCATCAAATGACCTCTTTTCTTCTGCTTTTTGCCTCCATCCAG
AAGTGCATCTTTCAGTCCTTCACTCCGAGGGGCACCTTCGAAGGGGGTGACGTCACTGACGGACACGGTG
ACTGACAGGCTGCCCTGGCTCAGTTATCACAGTGCTGATGCTGTCCATTCTAAAGGTACAGTACTGTGAT
AACTGAAGGATGGCAGCCATCTTGCCTTCCTCCCGAGAAGTCTCATCACAGCAGGCGAAAGGCATGGGAA
GCAGGACACCGGGACAGGACCAGACACACTTCACACCGCTCCTGTGTTCACCACTCTTCCAGGCTCCATT
TGGTAACAAGGAGCAAAACAATGTAGCAGCTTGCACAGGGTATGAAGACGGTTATGCTGAGGACAAACAG
GGTCTGTGGAACCTTTCATGTCTGGGAAGATTGACACTAAGAAAGTCCTAAGGACAAATCAACATATCTC
AAACGGAGAGAAACGATCCAT
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000148
Synonyms Mirn101 miR-101 Mirn101a
Locus ID 387143
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Documents
Resources

file-alt Citations

HIV-1 Tat C modulates expression of miRNA-101 to suppress VE-cadherin in human brain microvascular endothelial cells
Mishra, Singh
J. Neurosci. (2013) 33 (14), 5992-6000 DOI: 10.1523/JNEUROSCI.4796-12.2013
AI Summary: In this study, the OriGene pCMV-Mir miR-101 construct (#SC400013) was used to overexpress miR-101 in human brain microvascular endothelial cells, enabling the authors to investigate how miR-101 regulates VE-cadherin expression and contributes to blood-brain barrier disruption by HIV-1 Tat C.
Snippet: The CDH5 3′UTR construct in pMirTarget (#HmiT000052-MT01;GeneCopeia) and miR-101 construct as pCMV-Mir (#SC400013;Origene) were used in ... A 3.0 μm pore PTFE membrane insert (12-well plate;#3494;Corning Life Sciences) was used in the permeability assays.
Showing 1-1 of 1 papers.
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