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MirLet7c-1 Mouse MicroRNA Expression Plasmid (MI0000559)

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SKU
SC400710
Expression plasmid for mouse microRNA Let7c-1
  $424.00
In Stock*

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Specifications
Specifications
Product Data
Species Mouse
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UGUGUGCAUCCGGGUUGAGGUAGUAGGUUGUAUGGUUUAGAGUUACACCCUGGGAGUUAACUGUACAACCUUCUAGCUUUCCUUGGAGCACACU:
Mature Sequence: UGAGGUAGUAGGUUGUAUGGUU:
Full Insert
>MI0000559 GTTACAGTTGAGCCAGTTTCTCTTCAAAATCAAGGCATTATTCTACTTTTAAATTTGCTCGCTGCTAATG
GAAGTGTGGTCAAGGAGATGTTAGAAGACACTTCATGTGACCTATACTGTAAACTCACCAGATTAGGGTA
GTCCTGTAAGCTACAACTGAAGTTGATTTGTAAACTGCTAGAATATTCTATCTACAACCTTGCCAAGCCC
TTAGGTGTTTGGCCGCCATATTTGGAGGAGCGGACTGAAGATATGATAAGGAGTTTGAAGAAACATTGGA
AGCTGTGTGCATCCGGGTTGAGGTAGTAGGTTGTATGGTTTAGAGTTACACCCTGGGAGTTAACTGTACA
ACCTTCTAGCTTTCCTTGGAGCACACTTGAGCCATCGAGGAATTCTCCATCTCTTTAATCTGACTGAGCC
AATTTCTGTTCAAGAAAGTAATGTGTCATGAGTCCATGAGGCCTTAATGCATTACCTGATCATTCCAGAC
CTGATGGTTAGGATTCTGCTTGTGTCTACTTGTAAGCAATGACACAACGCTATTTCTCACGGGCTGTTAT
TTTGTGTTTACCAGTCTAAAGAACAAATGGGTAGTGAATTAAGTTGCATTAATATTGAAACTTTGGTTTC
TTTCATCAATGGAATCACAAAATTTGGCA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000559
Synonyms Mirnlet7c Mirnlet7c-1
Locus ID 387246
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Documents
Resources

file-alt Citations

Feedback repression of PPARα signaling by Let-7 microRNA
Yagai, Yan, Luo et al
Cell Rep (2021) 36 (6), 109506 DOI: 10.1016/j.celrep.2021.109506
Cited by 19
AI Summary: OriGene’s MirLet7c-1 expression plasmid was used as a template to amplify the pre-let-7c-1 sequence for generating AAV vectors, enabling functional studies of let-7c-1 in the reported research.
Snippet: d Stu I restriction enzymes. For pre-let-7c-1 expression AAV, the 470 bp mouse genomic sequence around pre-let-7c-1 was amplified using custom primers (Table S3) from a MirLet7c-1 expression plasmid (Origene). The amplified fragments were cloned into pscAAV-ALBp-EGFP plasmid with Not I, Stu I, and EcoR V restriction enzymes. The plasmids were transfected into HEK293T cells to produce AAV8 vectors
Showing 1-1 of 1 papers.
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