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MIR96 Human MicroRNA Expression Plasmid (MI0000098)

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SKU
SC400703
Expression plasmid for human microRNA MIR96
  $424.00
In Stock*

Frequently Bought Together (1)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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Other products for "MIR96 or MI0000098"

Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UGGCCGAUUUUGGCACUAGCACAUUUUUGCUUGUGUCUCUCCGCUCUGAGCAAUCAUGUGCAGUGCCAAUAUGGGAAA:
Mature Sequence: UUUGGCACUAGCACAUUUUUGCU:
Full Insert
>MI0000098 TAGGAGCAGGGAACGGGCATCGTGGGCCGCTGGTCTCTCCGCAGGGTCGGCAGGCCGCAGAGTGTGACTC
CTGTTCTGTGTATGGCACTGGTAGAATTCACTGTGAACAGTCTCAGTCAGTGAATTACCGAAGGGCCATA
AACAGAGCAGAGACAGATCCACGAGGGCCTCCGGAGCACCTTACCCACTTCTGCCTTGAGTGCTCCTAGA
CGTCGGAAACAGGCTGCTTCCAAGGGTGCAGGGATGCAAGGCCCCTCGTCCAGTGTGTCCCCAGAGAGCC
CGCACCAGTGCCATCTGCTTGGCCGATTTTGGCACTAGCACATTTTTGCTTGTGTCTCTCCGCTCTGAGC
AATCATGTGCAGTGCCAATATGGGAAAAGCAGGACCCGCAGCTGCGTCCGCCTCCCCTGCATCCTTGTGT
CAGGGCCCCAGCCTGCTCCTCCTCAAGGCCTCCTCACCGCCTCCCCAGCCCATCTGGCTCAGCTGCTGTG
TGAGGGCCCAGCGCTGGTGGGCAGCCAGATCGCCTTACACTGCCTGGGGCCACGGTAGAGCTGGGAGCCC
AGCAATCTGAGCTGGGCCAGCAGATGGGGCCGCCCAGGGCAGAGGTGGGGGAGTCTGAAACCATCTGTAG
GGCCATCCTGAATGGTGCCGTGGGTTGGAAA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains MI0000273
Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000098
Synonyms DFNA50 MIRN96 miR-96 miRNA96 hsa-mir-96
Locus ID 407053
Reviews
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Documents
Resources

file-alt Citations

miR-23b and miR-218 silencing increase Muscleblind-like expression and alleviate myotonic dystrophy phenotypes in mammalian models
Cerro-Herreros, Sabater-Arcis, Fernandez-Costa et al
Nat Commun (2018) 9 (1), 2482 DOI: 10.1038/s41467-018-04892-4
AI Summary: OriGene’s pCMV-MIR-GFP vector and miRNA plasmids were used to overexpress specific miRNAs in HeLa cells, enabling the authors to identify and validate miR-23b and miR-218 as regulators of Muscleblind-like proteins in their study of myotonic dystrophy.
Snippet: the miRNA overexpression assay, HeLa cells were seeded in 6-well plates at approximately 80% confluence and transfected with 1 µg of miRNA precursor sequences cloned into the pCMV-MIR-GFP vector (OriGene). At 48 h post-transfection, the cells were harvested for the qRT-PCR and western blot analyses. For the reporter assays, the cells were seeded in 24-well plates at approximately 80% conflue;For e...
FigureFigureFigure
Showing 1-1 of 1 papers.
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