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MIR363 Human MicroRNA Expression Plasmid (MI0000764)

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SKU
SC400361
Expression plasmid for human microRNA MIR363
  $424.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UGUUGUCGGGUGGAUCACGAUGCAAUUUUGAUGAGUAUCAUAGGAGAAAAAUUGCACGGUAUCCAUCUGUAAACC:
Mature Sequence: CGGGUGGAUCACGAUGCAAUUU:
Full Insert
>MI0000764 GTTTTGCAGGTTTGCATTTCAGCGTATATATGTATATGTGGCTGTGCAAATCCATGCAAAACTGATTGTG
ATAATGTGTGCTTCCTACGTCTGTGTGAACACACCTTCATGCGTATCTCCAGCACTCATGCCCATTCATC
CCTGGGTGGGGATTTGTTGCATTACTTGTGTTCTATATAAAGTATTGCACTTGTCCCGGCCTGTGGAAGA
AAGGAGGATTTTTATCGTCTTCTTATTTTAACTTTTAAAAGCCGTAAGTTCTGATATTTAGTCATTGTAA
AATGATCTGTTTTGCTGTTGTCGGGTGGATCACGATGCAATTTTGATGAGTATCATAGGAGAAAAATTGC
ACGGTATCCATCTGTAAACCGCAGGACCTTTGTTGGCGACATTCCTGATCAGCGCTACAGTAAGCTAGAT
GGTAAAAAATCCTTTCTCTAGTTGCTGCTATTAAAAAATAATTTGAAATATATTTGGATTTCTAAAAACT
ATGATAGCTAATTTATAAAGTATGTGTTTTGGAACTTTGGTGAATGATCAGATTGTTACTGAATGTGAAT
GAACCTCAAAATAAATGAAATTTATAGATCTGAAAAAATGACTCTTTTTTTTTTAAAAAAAATAATTAGC
AATGCTAGAGGAGTTTGTAAAATATCTCTTGGG
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains MI0000075
Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000764
Synonyms MIRN363 hsa-mir-363
Locus ID 574031
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Documents
Resources

file-alt Citations

Interferon-induced IFIT5 promotes epithelial-to-mesenchymal transition leading to renal cancer invasion
Lo, Bao, Cen et al
Am J Clin Exp Urol (2019) 7 (1), 31-45
AI Summary: OriGene’s miR-363 expression plasmid was used in this study to investigate the role of specific microRNAs in IFIT5-mediated epithelial-to-mesenchymal transition and renal cancer cell invasion.
Snippet: Each experiment was performed in triplicates. Construction of SSMut and DSMut pre-miRNA-expression plasmid. miR-363 and miR-128 expression plasmid was initially purchased from Origene and Genecopoeia, respectively
IFN-γ induces epithelial-to-mesenchymal transition of cancer cells via an unique microRNA processing
Hsieh, Lo, Pong et al
bioRxiv (2018)
Snippet: The pCMV-miR-363 expression plasmid initially purchased from Origene contains both premiR-92a-2 and pre-miR-363 sequence. In order to isolate pre-miR-363 sequence, we generated a Nhel cut site in the 5’-end of pre-miR-92a-2 using site-directed mutagenesis and excised pre-miR-363 sequence by using both NheI and MluI enzyme then inserted into the pCMV-miRNA empty vector to generate miR-363 expressio...
Showing 1-2 of 2 papers.
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