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MIRLET7B Human MicroRNA Expression Plasmid (MI0000063)

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SKU
SC400004
Expression plasmid for human microRNA LET7B
  $424.00
In Stock*

Frequently Bought Together (1)

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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: CGGGGUGAGGUAGUAGGUUGUGUGGUUUCAGGGCAGUGAUGUUGCCCCUCGGAAGAUAACUAUACAACCUACUGCCUUCCCUG:
Mature Sequence: UGAGGUAGUAGGUUGUGUGGUU:
Full Insert
>MI0000063 AAGGCACCTTTCCTGAGCAGGAAGTGAGAGGAACAGCTCTGCATACACTGGGTCCCACATGGCACAATCT
GAAGGCAGACAGTGGCTCCTCTGTACCTGGGGAAACTGAGGCCCAGAGAGCCAGGGACTTCCCAAGACCA
GCCAGCAGCAGCTGCCCCTTCCTGGGGTGCCATCTCCCCTGTCCCTCCTGCCCTGCGCCTGCCCAGCCCT
CCTGCTCTGGTGACTGAGGACCGCCAGGCAGGGGCTGGTGCTGGGCGGGGGGCGGCGGGCCCTCCCGCAG
TGCAAGGCCGGGCCTGGCGGGGTGAGGTAGTAGGTTGTGTGGTTTCAGGGCAGTGATGTTGCCCCTCGGA
AGATAACTATACAACCTACTGCCTTCCCTGAGGAGCCCAGTGACACGACCCCATGGGAGGGCCGCCCCCT
ACCTCAGTGACACGACCCCACGGGAGGGCTGCCCCCCACCTCAGTGACCTGCAGGGGGCCTGAGCCGAAG
CTGGGTGGGCATCTGGGAGCTAGATTCAATAAAGCTGTTCTGACCATGAACTTGGAACTGGCCCCTTTCA
TTCTGGAAGCCCAGGGACTGGGCTCTGGGGCGCGTCTGCTGCGGGACAGTGTGGAGGCCCCTGGCCACGA
GCTGTTCCAGGTCATGAGACTTGTCTGCTC
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000063
Synonyms LET7B let-7b MIRNLET7B hsa-let-7b
Locus ID 406884
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Documents
Resources

file-alt Citations

The Jumanjii Histone Demethylase KDM2B controls EZH2 Expression In Myelodysplastic Syndromes (MDS) Via Mir Let-7b (let7b), a Pathway That Is Bypassed By
Byon, Yeung, Deeg
Blood (2013) 122 (21), 1543
AI Summary: OriGene’s pre-BAB-let7b product was used in this study to overexpress let7b, enabling the authors to investigate its role in regulating EZH2 expression in myelodysplastic syndromes.
Snippet: miRZip plasmids (miRZip anti-sense) were used to knock-down let7b;pre-BAB-let7b (origene) served to overexpress let7b. Gene expression, protein levels and cell proliferation were analyzed by RQ-PCR, western blot and BrdU uptake. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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