DOT1L (NM_032482) Human Recombinant Protein

SKU TP762294

Purified recombinant protein of Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), Met1-Asp434, with N-terminal His tag, expressed in E.coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region(Met1-Asp434) of DOT1L
• Tag: N-His
• Predicted MW: 49.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_115871
• Locus ID: 84444
• UniProt ID: Q8TEK3
• Cytogenetics: 19p13.3
• RefSeq Size: 4889
• RefSeq ORF: 4611
• Synonyms: DOT1; KMT4
• Summary: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation