SPDYA (NM_182756) Human Recombinant Protein

SKU TP761262

Purified recombinant protein of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length SPDYA
• Tag: N-His
• Predicted MW: 36.3 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_877433
• Locus ID: 245711
• UniProt ID: Q5MJ70
• Cytogenetics: 2p23.2
• RefSeq Size: 1830
• RefSeq ORF: 939
• Synonyms: RINGO3; RINGOA; SPDY1; SPY1
• Summary: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).[UniProtKB/Swiss-Prot Function]
• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation