CD62P (SELP) Human Recombinant Protein

SKU
TP723989
Recombinant human SELP protein with C-terminal human Fc tag
  $770.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
SELP(Trp42-Aal771) hFc(Glu99-Ala330)
Tag C-Human Fc Tag
Predicted MW The protein has a predicted molecular mass of 106.1 kDa after removal of the signal peptide.The apparent molecular mass of SELP-hFc is approximately 130-150 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Storage Store the lyophilized protein at -20°C.
After reconstitution, store the protein at -80°C for 12 months.
Avoid repeated freezing and thawing.
Stability 12 months from date of despatch
Shipping Ambient
Reference Data
Locus ID 6403
UniProt ID P16109
Synonyms P-Selectin;CD62P;SELP;GMP-140
Summary This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
Protein Categories CD Markers, Cytokines, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
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Other "CD62P" proteins (4)
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PH309822 SELP MS Standard C13 and N15-labeled recombinant protein (NP_002996) 10 ug
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LC418960 Lyophilized SELP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY418960 Transient overexpression lysate of selectin P (granule membrane protein 140kDa, antigen CD62) (SELP) (as WB positive control) 100 ug
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TP309822 Recombinant protein of human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), 20 µg 20 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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