CD28 Human Recombinant Protein

SKU
TP723937
Recombinant human CD28 protein with C-terminal mouse Fc and 6×His tag
  $905.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His
Tag C-Mouse Fc and 6×His Tag
Predicted MW The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Recommended Dilution ELISA, Flow Cyt
Storage Store the lyophilized protein at -20°C.
After reconstitution, store the protein at -80°C for 12 months.
Avoid repeated freezing and thawing.
Stability 12 months from date of despatch
Shipping Ambient
Reference Data
Locus ID 940
UniProt ID P10747
Synonyms CD28;Tp44
Summary The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Protein Categories CD Markers, Cytokines, Membrane Proteins
Protein Families ELISA, Flow Cyt
Protein Pathways Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, T cell receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis
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Other "CD28" proteins (4)
SKU Description Size Price
LC401849 Lyophilized CD28 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401849 Transient overexpression lysate of CD28 molecule (CD28) (as WB positive control) 100 ug
$436.00
TP700222 Purified recombinant protein of human CD28 molecule (CD28), with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP700223 Purified recombinant protein of Human CD28 molecule (CD28), with C-terminal Fc tag, expressed in human cells 20 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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