CD47 Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Mouse Fc and 6×His Tag |
| Predicted MW | The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Buffer | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization |
| Reconstitution Method | 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample. |
| Preparation | Affinity purification |
| Recommended Dilution | ELISA |
| Storage | Store the lyophilized protein at -20°C. After reconstitution, store the protein at -80°C for 12 months. Avoid repeated freezing and thawing. |
| Stability | 12 months from date of despatch |
| Shipping | Ambient |
| Reference Data | |
| Locus ID | 961 |
| UniProt ID | Q08722 |
| Synonyms | CD47;MER6;IAP;OA3 |
| Summary | Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. |
| Protein Categories | ADC Targets, CAR-T Targets, CD Markers, Intracellular Proteins, Membrane Proteins |
| Protein Families | ELISA |
| Protein Pathways | ECM-receptor interaction |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CD47" proteins (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC404792 | Lyophilized CD47 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC419754 | Lyophilized CD47 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY404792 | Transient overexpression lysate of CD47 molecule (CD47), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY419754 | Transient overexpression lysate of CD47 molecule (CD47), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP700196 | Purified recombinant protein of Homo sapiens CD47 molecule (CD47), residues 19-141aa, with C-terminal Fc tag, expressed in HEK293 cells. | 20 ug |
$900.00
|
|
| TP700197 | Purified recombinant protein of Homo sapiens CD47 molecule (CD47), residues 19-141aa, with C-terminal DDK/His tag, expressed in HEK293 cells. | 20 ug |
$900.00
|
|
| TP710231 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 1, residues 19-141aa, with C-terminal DDK tag, expressed in sf9, 20ug | 20 ug |
$540.00
|
|
| TP720635 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 2 | 10 ug |
$235.00
|
|
| TP720959 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 2 | 10 ug |
$340.00
|
|
Citations
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