Ccl3 (NM_011337) Mouse Recombinant Protein

CAT#: TP723888

Purified recombinant protein of Mouse chemokine (C-C motif) ligand 3 (Ccl3 / MIP-1a)

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse CCL3, the region of Ala24 - Ala92, from gene Accession# NM_011337
• Tag: Tag Free
• Predicted MW: 7.8 kDa
• Concentration: lot specific
• Purity: >97%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM Glycine, 150 mM NaCl, pH 3.0
• Bioactivity: Recombinant mouse CCL3 (MIP-1α) chemoattracts Baf3-hCCR5 transfectants in a dose-dependent manner. The ED50 for this effect is 0.1– 1.0 ng/mL.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_035467
• Locus ID: 20302
• UniProt ID: P10855, Q5QNW0
• Cytogenetics: 11 51.04 cM
• Refseq Size: 804
• Refseq ORF: 279
• Synonyms: AI323804; G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3
• Summary: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.[UniProtKB/Swiss-Prot Function]